CAJ | 학술논문

目的：探讨帕金森病（ PD）前期多巴胺能神经元受损至出现局部病理改变前脑部葡萄糖代谢的改变。方法腹腔注射1－甲基－4－苯基－1，2，3，6四氢吡啶（ MPTP）制作慢性PD小鼠模型，对照组注射生理盐水。于不同时间点，PET成像检测小鼠脑黑质及纹状体葡萄糖代谢的变化，蛋白电泳检测黑质酪氨酸羟化酶（ TH）表达。结果小鼠双侧纹状体出现葡萄糖代谢降低时黑质致密部TH和磷酸化TH表达水平并无明显改变。结论PD小鼠模型双侧纹状体葡萄糖代谢降低早于黑质TH的下降。
목적：탐토파금삼병（ PD）전기다파알능신경원수손지출현국부병리개변전뇌부포도당대사적개변。방법복강주사1－갑기－4－분기－1，2，3，6사경필정（ MPTP）제작만성PD소서모형，대조조주사생리염수。우불동시간점，PET성상검측소서뇌흑질급문상체포도당대사적변화，단백전영검측흑질락안산간화매（ TH）표체。결과소서쌍측문상체출현포도당대사강저시흑질치밀부TH화린산화TH표체수평병무명현개변。결론PD소서모형쌍측문상체포도당대사강저조우흑질TH적하강。
Objective To investigate the changes of brain glucose metabolism in mice at the early stage of Parkin-son′s disease ( PD) when local pathological disorders does not appear .Mtehods A chronic PD model was established through intraperitoneal injection of MPTP into mice , while control mice were injected with normal saline .The changes of glucose metabolism in the substantia nigra and striatum were detected by PET imaging .The amounts of tyrosine hydroxy-lase ( TH) and phosphor -TH ( p-TH) in the substantial nigra were measured by Western blotting .Rseult s There was no remarkable change in the amounts of TH and p -TH in the substantial nigra when the glucose metabolism in the bilateral striatum of mice became weakened .Conclusion The weakening of glucose metabolism in the bilateral striatum appears before the levels of TH and p -TH are reduced in the substantial nigra .