CAJ | 학술논문

目的：探讨甘草酸（glycyrrhizin，Gly）对1型糖尿病（T1DM）脑缺血／再灌注损伤大鼠神经功能恢复的影响。方法应用链脲佐菌素（streptozotocin，STZ）诱导建立雄性SD大鼠1型糖尿病模型，诱导14天后进行大脑中动脉缺血／再灌注损伤（middle cerebral artery occlusion ，MCAo）模型制备。脑缺血／再灌注后24 h，分别经尾静脉给予PBS、Gly 1 ml（4 mg／kg）。实验大鼠随机分为3组（每组n＝6）：正常大鼠脑缺血／再灌注对照组（ N－MCAo组）、T1DM大鼠脑缺血／再灌注损伤组（ T1DM－MCAo组）、T1DM大鼠脑缺血／再灌注损伤Gly治疗组（ T1DM－MCAo＋Gly组）。术后1、7、14天分别对各组大鼠进行神经功能损伤评估。结果术后第1天，各组改良神经功能缺损评分（mNSS）、足错步率比较差异无统计学意义（P＞0．05）；术后第7、14天，与N－MCAo组相比，T1DM－MCAo组mNSS明显升高（P＜0．05）；与T1DM－MCAo组相比，T1DM－MCAo＋Gly 组大鼠mNSS明显降低（P＜0．05）。结论 Gly促进T1DM脑缺血／再灌注损伤大鼠神经功能恢复。
목적：탐토감초산（glycyrrhizin，Gly）대1형당뇨병（T1DM）뇌결혈／재관주손상대서신경공능회복적영향。방법응용련뇨좌균소（streptozotocin，STZ）유도건립웅성SD대서1형당뇨병모형，유도14천후진행대뇌중동맥결혈／재관주손상（middle cerebral artery occlusion ，MCAo）모형제비。뇌결혈／재관주후24 h，분별경미정맥급여PBS、Gly 1 ml（4 mg／kg）。실험대서수궤분위3조（매조n＝6）：정상대서뇌결혈／재관주대조조（ N－MCAo조）、T1DM대서뇌결혈／재관주손상조（ T1DM－MCAo조）、T1DM대서뇌결혈／재관주손상Gly치료조（ T1DM－MCAo＋Gly조）。술후1、7、14천분별대각조대서진행신경공능손상평고。결과술후제1천，각조개량신경공능결손평분（mNSS）、족착보솔비교차이무통계학의의（P＞0．05）；술후제7、14천，여N－MCAo조상비，T1DM－MCAo조mNSS명현승고（P＜0．05）；여T1DM－MCAo조상비，T1DM－MCAo＋Gly 조대서mNSS명현강저（P＜0．05）。결론 Gly촉진T1DM뇌결혈／재관주손상대서신경공능회복。
Objective To investigate the effects of glycyrrhizin ( Gly) on the restoration of nervous functions in type 1 diabetic rats with cerebral ischemia/reperfusion injury .Methods A model of type 1 diabetic rats was established through injection of streptozotocin .Two weeks later , all rats were subjected to middle cerebral artery occlusion ( MCAo) for 2 h.One day later, the rats were injected with 1 ml of PBS or Gly (4 mg/kg) via the tail vein.The rats were divided into three groups (n=6):a normal control group (Group N-MCAo), a MCAo group (Group T1Dm-MCAo) and a Gly treatment group(Group T1DM-MCAo+Gly).Then, the nervous functional injuries in rats were assessed on Days 1, 7and 14 after operation.Results No statistical difference was measured as to the modified Neurological Severity Score (mNSS) and wrong step rate among the groups on Day 1 (P >0.05).On Days 7 and 14, Group T1DM-MCAo produced substantial decreases in mNSS than Group N -MCAo (P<0.05), which were reduced after Gly treatment (P<0.05).Conclusion Gly treatment can stimulate the recovery of functional outcomes in type 1 diabetic rats with ische-mia/reperfusion injury in brain .