现代肿瘤医学
現代腫瘤醫學
현대종류의학
Journal of Modern Oncology
2015年
19期
2804-2809
,共6页
赵滢%宿文辉%张天彪%王强
趙瀅%宿文輝%張天彪%王彊
조형%숙문휘%장천표%왕강
S100 钙结合蛋白 A2%胃癌%免疫组化%RT - PCR%Western bolt
S100 鈣結閤蛋白 A2%胃癌%免疫組化%RT - PCR%Western bolt
S100 개결합단백 A2%위암%면역조화%RT - PCR%Western bolt
S100 calcium binding protein A2%gastric cancer%immunohistochemistry%RT - PCR%Western bolt
目的：探讨 S100A2在慢性胃炎、肠上皮化生和胃癌组织样本中的表达及与胃癌临床病理特征及预后的关系。方法：利用 HE 染色对所取胃组织标本进行病理组织学诊断；采用免疫组织化学方法检测标本S100A2蛋白的表达；qRT - PCR 法检测 S100A2 mRNA 表达；Western blot 检测 S100A2蛋白的表达；采用 Kap-lan - Meier 分析累积生存率。结果：应用 qRT - PCR 方法分析不同胃组织标本 S100A2 mRNA 的表达水平，发现 S100A2 mRNA 的表达水平按以下顺序递减：胃炎，肠上皮化生，胃癌（ P ＜0.001），与 Western bolt 分析S100A2蛋白的表达评定结果相吻合。免疫组化分析：S100A2在胃炎、肠上皮化生和胃癌组织细胞质中表达， S100A2阳性表达率为胃炎100％（73/73），肠上皮化生90.7％（78/86），胃癌48.9％（170/348）（P ＜0.001）。在胃癌中，S100A2蛋白的表达缺失在青年患者中多于老年患者。S100A2蛋白的表达与肿瘤大小、浸润深度、淋巴管和静脉浸润、淋巴结转移、肿瘤 TNM 分期呈负相关，并随胃癌分化程度降低，在高分化癌、中分化癌和低分化癌中，S100A2的阳性表达率分别为65.9％、56.4％和17.2％（P ＜0.05），和患者性别无相关性（ P ＞0.05）。Kaplan - Meier 分析累积生存率显示：弱或中度 S100A2基因表达的患者累积生存率明显高于不表达S100A2的患者。多因素分析显示 S100A2表达、浸润深度、淋巴管及静脉浸润、淋巴结转移、TNM 分期是胃癌患者预后的独立因素。结论：在胃炎-肠上皮化生-胃癌过程中 S100A2 mRNA 和蛋白表达逐渐降低。S100A2表达与肿瘤大小、浸袭深度、淋巴管和静脉侵犯、淋巴结转移及 TNM 分期负相关，并随胃癌分化程度降低，S100A2的阳性表达率逐渐降低。S100A2表达是独立的胃癌预后预测因素，是胃癌患者预后良好的指标之一。
目的：探討 S100A2在慢性胃炎、腸上皮化生和胃癌組織樣本中的錶達及與胃癌臨床病理特徵及預後的關繫。方法：利用 HE 染色對所取胃組織標本進行病理組織學診斷；採用免疫組織化學方法檢測標本S100A2蛋白的錶達；qRT - PCR 法檢測 S100A2 mRNA 錶達；Western blot 檢測 S100A2蛋白的錶達；採用 Kap-lan - Meier 分析纍積生存率。結果：應用 qRT - PCR 方法分析不同胃組織標本 S100A2 mRNA 的錶達水平，髮現 S100A2 mRNA 的錶達水平按以下順序遞減：胃炎，腸上皮化生，胃癌（ P ＜0.001），與 Western bolt 分析S100A2蛋白的錶達評定結果相吻閤。免疫組化分析：S100A2在胃炎、腸上皮化生和胃癌組織細胞質中錶達， S100A2暘性錶達率為胃炎100％（73/73），腸上皮化生90.7％（78/86），胃癌48.9％（170/348）（P ＜0.001）。在胃癌中，S100A2蛋白的錶達缺失在青年患者中多于老年患者。S100A2蛋白的錶達與腫瘤大小、浸潤深度、淋巴管和靜脈浸潤、淋巴結轉移、腫瘤 TNM 分期呈負相關，併隨胃癌分化程度降低，在高分化癌、中分化癌和低分化癌中，S100A2的暘性錶達率分彆為65.9％、56.4％和17.2％（P ＜0.05），和患者性彆無相關性（ P ＞0.05）。Kaplan - Meier 分析纍積生存率顯示：弱或中度 S100A2基因錶達的患者纍積生存率明顯高于不錶達S100A2的患者。多因素分析顯示 S100A2錶達、浸潤深度、淋巴管及靜脈浸潤、淋巴結轉移、TNM 分期是胃癌患者預後的獨立因素。結論：在胃炎-腸上皮化生-胃癌過程中 S100A2 mRNA 和蛋白錶達逐漸降低。S100A2錶達與腫瘤大小、浸襲深度、淋巴管和靜脈侵犯、淋巴結轉移及 TNM 分期負相關，併隨胃癌分化程度降低，S100A2的暘性錶達率逐漸降低。S100A2錶達是獨立的胃癌預後預測因素，是胃癌患者預後良好的指標之一。
목적：탐토 S100A2재만성위염、장상피화생화위암조직양본중적표체급여위암림상병리특정급예후적관계。방법：이용 HE 염색대소취위조직표본진행병리조직학진단；채용면역조직화학방법검측표본S100A2단백적표체；qRT - PCR 법검측 S100A2 mRNA 표체；Western blot 검측 S100A2단백적표체；채용 Kap-lan - Meier 분석루적생존솔。결과：응용 qRT - PCR 방법분석불동위조직표본 S100A2 mRNA 적표체수평，발현 S100A2 mRNA 적표체수평안이하순서체감：위염，장상피화생，위암（ P ＜0.001），여 Western bolt 분석S100A2단백적표체평정결과상문합。면역조화분석：S100A2재위염、장상피화생화위암조직세포질중표체， S100A2양성표체솔위위염100％（73/73），장상피화생90.7％（78/86），위암48.9％（170/348）（P ＜0.001）。재위암중，S100A2단백적표체결실재청년환자중다우노년환자。S100A2단백적표체여종류대소、침윤심도、림파관화정맥침윤、림파결전이、종류 TNM 분기정부상관，병수위암분화정도강저，재고분화암、중분화암화저분화암중，S100A2적양성표체솔분별위65.9％、56.4％화17.2％（P ＜0.05），화환자성별무상관성（ P ＞0.05）。Kaplan - Meier 분석루적생존솔현시：약혹중도 S100A2기인표체적환자루적생존솔명현고우불표체S100A2적환자。다인소분석현시 S100A2표체、침윤심도、림파관급정맥침윤、림파결전이、TNM 분기시위암환자예후적독립인소。결론：재위염-장상피화생-위암과정중 S100A2 mRNA 화단백표체축점강저。S100A2표체여종류대소、침습심도、림파관화정맥침범、림파결전이급 TNM 분기부상관，병수위암분화정도강저，S100A2적양성표체솔축점강저。S100A2표체시독립적위암예후예측인소，시위암환자예후량호적지표지일。
Objective:To study the relationship between S100A2 expressiol in chronic gastritis,intestinal metapla-sia,gastric cancer and clinical characteristics. Methods:HE staining was used for gastric specimens histopathological diagnosis. Using immunohistochemistry to detect the expression of tissue S100A2 protein. qRT - PCR was used to de-tect mRNA expression of S100A2 gene. Western blot for S100A2 gene encoding protein. Kaplan - Meier survival curves were used to distinguish and compare survival. Results:S100A2,S100A2 mRNA expression levels were in the following descending order:Gastritis,intestinal metaplasia and carcinoma(P < 0. 001). Immunohistochemical analysis of S100A2 expression:S100A2 was detected in gastritis(Gas),intestinal metaplasia(IM)and cancer(Ca)cytoplas-mic expression. Overall,S100 A2 expression results for gastritis cases 100%(73 / 73),90. 7% IM cases(78 /86),48. 9% of gastric cancer cases(170 / 348)(P < 0. 001). In gastric cancer,the lack of S100A2 protein in elderly patients than in young patients. S100A2 protein expression and tumor size,depth of invasion,lymphatic and venous in-vasion,lymph node metastasis,TNM stage in cancer patients was negatively correlated,the positive rate of S100A2 ex-pression were 65. 9% in highly,56. 4% in moderately and 17. 2% in poorly differentiated gastric carcinoma respec-tively(P < 0. 05),but no correlation between gender and patients(P > 0. 05). Kaplan - Meier analysis of cumulative survival showed:Weak or moderate expressions of S100A2 gene survival were significantly higher than patients without S100A2 expression. Multivariate analysis showed that,S100 A2 expression,depth of invasion,lymphatic and venous invasion,lymph node metastasis,TNM staging of gastric cancer were independent poor prognostic factors. Conclusion:The expression of S100A2 is a more favorable prognostic factor in patients with gastric cancer. Cox proportional haz-ards analysis,multivariate analysis showed that the depth of tumor invasion,lymphatic or venous invasion,lymph node metastasis,distant metastasis,TNM stage and S100A2 expression are independent prognostic factors in patients with gastric cancer. The positive rate of S100A2 expression decreased by the degree of differentiation of gastric carcinoma. These results suggest that S100A2 is an independent prognostic factor in gastric cancer,and can be used as a molecu-lar marker distinguished two types of cancer.
