CAJ | 학술논문

目的：探讨肿瘤干细胞标记物Nestin对肿瘤细胞抗凋亡的影响。方法采用骨肉瘤细胞MG63细胞株，经过0.9μmol／L顺铂处理12 h、洗涤后经免疫组化染色，显微镜观察幸存细胞的特性；并通过彗星实验验证0.9μmol／L顺铂对Nestin表达阳性细胞和Nestin表达阴性细胞的损伤程度差异。结果显微镜观察显示，顺铂处理后会有少量细胞幸存，且这些未凋亡的细胞绝大多数为Nestin表达阳性细胞；采用彗星实验的研究表明， Nestin表达阳性细胞经顺铂处理后的细胞核染色体损伤较Nestin表达阴性细胞更小。结论 Nestin可能在骨肉瘤MG63细胞抵抗化疗药物顺铂诱导的DNA损伤中起细胞核保护作用。
목적：탐토종류간세포표기물Nestin대종류세포항조망적영향。방법채용골육류세포MG63세포주，경과0.9μmol／L순박처리12 h、세조후경면역조화염색，현미경관찰행존세포적특성；병통과혜성실험험증0.9μmol／L순박대Nestin표체양성세포화Nestin표체음성세포적손상정도차이。결과현미경관찰현시，순박처리후회유소량세포행존，차저사미조망적세포절대다수위Nestin표체양성세포；채용혜성실험적연구표명， Nestin표체양성세포경순박처리후적세포핵염색체손상교Nestin표체음성세포경소。결론 Nestin가능재골육류MG63세포저항화료약물순박유도적DNA손상중기세포핵보호작용。
Objective To investigate the effect nestin, a marker of tumor stem cells, on the anti-apoptosis mech-anism of in tumor cells.Methods The osteosarcoma cell line MG63 was utilized for the following investigations.After 0.9μmol/L cisplatin treatment for 12 h and immunohistochemistry ( IHC) staining, the characteristics of surviving cells were observed under the microscope.Damage of 0.9μmol/L cisplatin treatment on nestin-positive cells and nestin-negative cells were compared using comet assay.Results Microscopic observation showed that a small amount of cells survived af-ter cisplatin treatment, the majority of which were nestin-positive.Comet assay demonstrated that damage of cisplatin treatment on nuclear chromosomes in nestin-positive cells was weaker than in nestin-negative cells.Conclusion In the osteosarcoma cell line MG63, nestin may exert a protective role on the nucleus and provide chemotherapy resistance in cisplatin-induced DNA damage.