河北渔业
河北漁業
하북어업
Hebei Fisheries
2015年
9期
4-6
,共3页
马睿霄%裴晨红%金枫清%佟长青%李伟
馬睿霄%裴晨紅%金楓清%佟長青%李偉
마예소%배신홍%금풍청%동장청%리위
双齿围沙蚕%浸膏%抑菌作用%血管紧张肽 I转化酶
雙齒圍沙蠶%浸膏%抑菌作用%血管緊張肽 I轉化酶
쌍치위사잠%침고%억균작용%혈관긴장태 I전화매
Perinereis aibuhitensis%extract%antibacterial activity%angiotensin converting enzyme (ACE)
用70％乙醇对双齿围沙蚕进行热浸提后，得到双齿围沙蚕浸膏。然后对得到的浸膏依次用石油醚、乙酸乙酯及正丁醇进行萃取，得到3部分萃取物。对双齿围沙蚕浸膏及各有机溶剂萃取部分进行抑菌试验和血管紧张肽I转化酶（ACE）抑制测试。结果表明，双齿围沙蚕浸膏及各有机溶剂萃取部分均具有抑制 ACE作用，且具有剂量依赖性。双齿围沙蚕石油醚萃取部分对枯草芽孢杆菌和大肠杆菌有抑菌活性，乙酸乙酯萃取部分对枯草芽孢杆菌、希瓦氏菌以及金黄色葡萄球菌具有抑菌活性，正丁醇萃取部分对希瓦氏菌具有抑菌活性。
用70％乙醇對雙齒圍沙蠶進行熱浸提後，得到雙齒圍沙蠶浸膏。然後對得到的浸膏依次用石油醚、乙痠乙酯及正丁醇進行萃取，得到3部分萃取物。對雙齒圍沙蠶浸膏及各有機溶劑萃取部分進行抑菌試驗和血管緊張肽I轉化酶（ACE）抑製測試。結果錶明，雙齒圍沙蠶浸膏及各有機溶劑萃取部分均具有抑製 ACE作用，且具有劑量依賴性。雙齒圍沙蠶石油醚萃取部分對枯草芽孢桿菌和大腸桿菌有抑菌活性，乙痠乙酯萃取部分對枯草芽孢桿菌、希瓦氏菌以及金黃色葡萄毬菌具有抑菌活性，正丁醇萃取部分對希瓦氏菌具有抑菌活性。
용70％을순대쌍치위사잠진행열침제후，득도쌍치위사잠침고。연후대득도적침고의차용석유미、을산을지급정정순진행췌취，득도3부분췌취물。대쌍치위사잠침고급각유궤용제췌취부분진행억균시험화혈관긴장태I전화매（ACE）억제측시。결과표명，쌍치위사잠침고급각유궤용제췌취부분균구유억제 ACE작용，차구유제량의뢰성。쌍치위사잠석유미췌취부분대고초아포간균화대장간균유억균활성，을산을지췌취부분대고초아포간균、희와씨균이급금황색포도구균구유억균활성，정정순췌취부분대희와씨균구유억균활성。
Perinereis aibuhitensis crude extract was obtained after P .aibuhitensis was exhaustively ex‐tracted by 70% hot ethanol .The resulting extract was extracted by petroleum ether ,ethyl acetate and n-butanol in turn ,resulting in 3 extracts from the organic solvent layer .The crude extract and the 3 extracts from the organic solvent layer were applying to inhibit the growth of bacteria and the activity of angiotensin converting enzyme (ACE) .The results showed that the extract from petroleum ether layer had the antibacterial activity with the Bacillus subtilis and Escherichia coli ,the extract from eth‐yl acetate layer had antibacterial activity with B .subtilis ,Shewanella sp .and Staphylococcus aureus , and the extract from n-butanol layer had antibacterial activity with Shewanella sp .The crude extract and organic solvent extracts had inhibition to ACE in a dose-dependent manner .
