CAJ | 학술논문

目的研究结核分枝杆菌( Mtb)耐药基因芯片法检测对初治菌阳肺结核治疗指导价值. 方法应用Mtb耐药基因芯片检测100例初治肺结核感染菌株对利福平( RFP)、异烟肼( INH)、链霉素( SM)、乙胺丁醇( EMB)的耐药性,并与BACTEC MGIT960系统检测结果比对,经鉴别罗氏培养基( PNB)除外非结核分枝杆菌( NTM) 结果 Mtb耐药基因芯片法检测耐RFP、INH、SM、EMB的敏感性和特异性分别为64. 29%、75. 00%、52. 94%、62. 5.%;93. 02%、82. 5%、93. 98%、97. 83%. 结论结核分枝杆菌耐药基因芯片对于5种基因相关突变所致耐药性的敏感性略低,特异性较高,对初治菌阳肺结核临床治疗有一定参考价值.
목적 연구결핵분지간균( Mtb)내약기인심편법검측대초치균양폐결핵치료지도개치. 방법응용Mtb내약기인심편검측100례초치폐결핵감염균주대리복평( RFP)、이연정( INH)、련매소( SM)、을알정순( EMB)적내약성,병여BACTEC MGIT960계통검측결과비대,경감별라씨배양기( PNB)제외비결핵분지간균( NTM) 결과 Mtb내약기인심편법검측내RFP、INH、SM、EMB적민감성화특이성분별위64. 29%、75. 00%、52. 94%、62. 5.%;93. 02%、82. 5%、93. 98%、97. 83%. 결론 결핵분지간균내약기인심편대우5충기인상관돌변소치내약성적민감성략저,특이성교고,대초치균양폐결핵림상치료유일정삼고개치.
Objective To study the value of Mycobacterium tuberculosis ( Mtb) drug resistance gene chip in guiding initial-treated sputum-positive pulmonary tuberculosis ( TB) . Methods 100 Mtb clinical isolates were col-lected from patients who were at stage of initial treated sputum positive pulmonary TB, and were examined by gene chip technique to detect the resistance to RFP, INH, SM and EMB. The results were compared with BACTEC MGIT960 ( except for NTM by PNB) . Results Taking BACTEC MGIT960 as the gold standard test, the sensitivity of gene chip test was 64. 29%, 75. 00%, 52. 94% and 62. 50%, and the specificity as 93. 02.%, 82. 5%, 93. 98% and 97. 83%, respectively for RFP-resistance, INH-resistance, SM-resistance, and EMB-resistance. Con-clusion Mtb gene chip shows slightly lower sensitivity and high specificity when testing the resistance to the five drugs. Therefore, it has certain reference value in guiding the initial treated sputum positive pulmonary tuberculosis.