苏州科技学院学报（自然科学版）
囌州科技學院學報（自然科學版）
소주과기학원학보（자연과학판）
Journal of Suzhou University of Science and Technology (Natural Science)
2015年
3期
36-42
,共7页
拙政园%微生物多样性%末端限制性片段长度多态性%底泥%土壤
拙政園%微生物多樣性%末耑限製性片段長度多態性%底泥%土壤
졸정완%미생물다양성%말단한제성편단장도다태성%저니%토양
Humble Administrator's Garden%microbial diversity%T-RFLP%sediment%soil
采集拙政园3个底泥和3个土壤样品，运用末端限制性片段长度多态性（T-RFLP）技术分析拙政园6个样品的微生物多样性。研究结果显示：底泥细菌中T-RFs片段大小为181 bp、211 bp的菌种为优势菌种，土壤细菌中T-RFs片段大小为91 bp、205 bp的菌种为优势菌种。各样点底泥的真菌种类各不相同，且种类较少，无明显的优势菌种，而土壤真菌中T-RFs片段大小为355 bp的菌种为优势菌种。这说明拙政园底泥、土壤的细菌和真菌多样性存在差异，细菌多样性明显高于真菌。为苏州园林微生物多样性及群落结构累积了有价值的资料。
採集拙政園3箇底泥和3箇土壤樣品，運用末耑限製性片段長度多態性（T-RFLP）技術分析拙政園6箇樣品的微生物多樣性。研究結果顯示：底泥細菌中T-RFs片段大小為181 bp、211 bp的菌種為優勢菌種，土壤細菌中T-RFs片段大小為91 bp、205 bp的菌種為優勢菌種。各樣點底泥的真菌種類各不相同，且種類較少，無明顯的優勢菌種，而土壤真菌中T-RFs片段大小為355 bp的菌種為優勢菌種。這說明拙政園底泥、土壤的細菌和真菌多樣性存在差異，細菌多樣性明顯高于真菌。為囌州園林微生物多樣性及群落結構纍積瞭有價值的資料。
채집졸정완3개저니화3개토양양품，운용말단한제성편단장도다태성（T-RFLP）기술분석졸정완6개양품적미생물다양성。연구결과현시：저니세균중T-RFs편단대소위181 bp、211 bp적균충위우세균충，토양세균중T-RFs편단대소위91 bp、205 bp적균충위우세균충。각양점저니적진균충류각불상동，차충류교소，무명현적우세균충，이토양진균중T-RFs편단대소위355 bp적균충위우세균충。저설명졸정완저니、토양적세균화진균다양성존재차이，세균다양성명현고우진균。위소주완림미생물다양성급군락결구루적료유개치적자료。
Terminal restriction fragment length polymorphism (T-RFLP) technique was used to analyze microbial diversity in 3 points of sediment and 3 points of soil in Humble Administrator's Garden. The results show that a-mong the sediment bacteria ,T-RFs fragments with the sizes of 181 bp ,211 bp were the dominant bacteria. A-mong soil bacteria ,T-RFs fragments with the sizes of 91 bp ,205 bp were the dominant bacteria. Among sediment fungi, species of each site were not identical. Among soil fungi ,T-RFs fragment with the size of 355 bp was the dominant fungus. Bacterial and fungal diversity of sediment and soil in Humble Administrator's Garden were dif-ferent. Bacterial diversity was significantly higher than that of fungi. This study has provided valuable data for microbial diversity and community structure of Suzhou gardens.
