海洋科学
海洋科學
해양과학
Marine Sciences
2015年
8期
7-15
,共9页
周爱国%谢少林%陈金涛%陈言峰%邹记兴
週愛國%謝少林%陳金濤%陳言峰%鄒記興
주애국%사소림%진금도%진언봉%추기흥
石斑鱼(Epinephelus)%核糖体DNA%ITS1%分子系统进化
石斑魚(Epinephelus)%覈糖體DNA%ITS1%分子繫統進化
석반어(Epinephelus)%핵당체DNA%ITS1%분자계통진화
Epinephelus%rDNA%ITS1%molecular phylogenetic relationship
为了明确分布于中国海域点带石斑鱼(Epinephelus malabaricus)、青石斑鱼(E. awoara)、巨石斑鱼(E. tauvina)、六带石斑鱼(E. sexfasciatus)和宝石石斑鱼(E. areolatus))等石斑鱼属(Epinephelus)鱼类的系统发育关系，作者采用PCR和DNA测序技术，测定了5种石斑鱼属鱼类的核糖体DNA ITS1序列，获得其ITS片段长度分别为537、536、535、528、532 bp。研究结果表明：5种石斑鱼rDNA ITS1序列的碱基组成趋势相似， A+T含量皆低于C+G含量， A+T含量最低为宝石石斑鱼(37.6％)，最高为六带石斑鱼(41.5%)；基于Kimura-2双参数模型计算得到上述石斑鱼种间遗传距离在0.0000~0.3002。基于MP法、ML法和NJ法构建的3种分子系统树表明， rDNA ITS1序列与形态呈同步进化关系，宝石石斑鱼与其他4种石斑鱼的亲缘关系较远，表明在所研究的物种中最早分化而出，而点带石斑鱼与青石斑鱼的亲缘关系最近，分化的时间最晚。
為瞭明確分佈于中國海域點帶石斑魚(Epinephelus malabaricus)、青石斑魚(E. awoara)、巨石斑魚(E. tauvina)、六帶石斑魚(E. sexfasciatus)和寶石石斑魚(E. areolatus))等石斑魚屬(Epinephelus)魚類的繫統髮育關繫，作者採用PCR和DNA測序技術，測定瞭5種石斑魚屬魚類的覈糖體DNA ITS1序列，穫得其ITS片段長度分彆為537、536、535、528、532 bp。研究結果錶明：5種石斑魚rDNA ITS1序列的堿基組成趨勢相似， A+T含量皆低于C+G含量， A+T含量最低為寶石石斑魚(37.6％)，最高為六帶石斑魚(41.5%)；基于Kimura-2雙參數模型計算得到上述石斑魚種間遺傳距離在0.0000~0.3002。基于MP法、ML法和NJ法構建的3種分子繫統樹錶明， rDNA ITS1序列與形態呈同步進化關繫，寶石石斑魚與其他4種石斑魚的親緣關繫較遠，錶明在所研究的物種中最早分化而齣，而點帶石斑魚與青石斑魚的親緣關繫最近，分化的時間最晚。
위료명학분포우중국해역점대석반어(Epinephelus malabaricus)、청석반어(E. awoara)、거석반어(E. tauvina)、륙대석반어(E. sexfasciatus)화보석석반어(E. areolatus))등석반어속(Epinephelus)어류적계통발육관계，작자채용PCR화DNA측서기술，측정료5충석반어속어류적핵당체DNA ITS1서렬，획득기ITS편단장도분별위537、536、535、528、532 bp。연구결과표명：5충석반어rDNA ITS1서렬적감기조성추세상사， A+T함량개저우C+G함량， A+T함량최저위보석석반어(37.6％)，최고위륙대석반어(41.5%)；기우Kimura-2쌍삼수모형계산득도상술석반어충간유전거리재0.0000~0.3002。기우MP법、ML법화NJ법구건적3충분자계통수표명， rDNA ITS1서렬여형태정동보진화관계，보석석반어여기타4충석반어적친연관계교원，표명재소연구적물충중최조분화이출，이점대석반어여청석반어적친연관계최근，분화적시간최만。
In order to explore the phylogenetic relationship among 5 endemic species of groupers,Epinephelus malabaricus, E. awoara,E. tauvina,E. sexfasctaius, andE. areolatus in China Sea. The sequences of rDNA ITS1 were analyzed and their lengths were determined to be 537, 536, 535, 528 and 532 bp respectively. The results were as follows: The basic composition of rDNA ITS1 sequences of 5 kinds of groupers was close to each other. The A+T component was lower than C+G component. A+T was the lowest (37.6%) inE. areolatus and the highest (41.5%) inE. sexfasctaius. The genetic distance measured using the kimura-two parameter model was from 0.0000 to 0.3002 in 5 kinds of groupers. The cluster result of 5 kinds of groupers in molecular phylogenetic trees constructed using MP, ML and NJ method were identical, suggesting that rDNA ITS1 sequences and morphological evolution might be synchronous. In 5 kinds of groupers,E. areolatus showed far away from other 4 species, suggesting that theE. areolatus might differentiated earlyfrom other species. WhileE. malabaricus showed nearest toE. awoara, suggesting that this species could be differentiated later.
