CAJ | 학술논문

浒苔(Ulva prolifera)是构成黄海绿潮的优势种，通过构建浒苔稳定转化系统，可以为相关基因功能验证提供有力工具。本文以浒苔原生质体再生苗为材料，试验了对除草剂草丁膦和6种抗生素的敏感性，发现浒苔对氨苄青霉素、卡那霉素、硫酸链霉素、Zeocin 4种抗生素不敏感，对氯霉素、潮霉素较敏感，对除草剂草丁膦极为敏感，进一步统计得出草丁膦对浒苔的半致死剂量(LD50)和95%可信限，发现敏感性与光周期明显相关。上述结果为进一步筛选转化子、构建浒苔稳定转化系统打下了基础。
호태(Ulva prolifera)시구성황해록조적우세충，통과구건호태은정전화계통，가이위상관기인공능험증제공유력공구。본문이호태원생질체재생묘위재료，시험료대제초제초정련화6충항생소적민감성，발현호태대안변청매소、잡나매소、류산련매소、Zeocin 4충항생소불민감，대록매소、조매소교민감，대제초제초정련겁위민감，진일보통계득출초정련대호태적반치사제량(LD50)화95%가신한，발현민감성여광주기명현상관。상술결과위진일보사선전화자、구건호태은정전화계통타하료기출。
Ulva proliferais the dominant species of green tides in the Yellow Sea, which can accumulate a large amount of biomass in a short period. Establishing the genetic transformation system ofU. proliferacan provide a powerful tool for verifying the function of related genes. In this paper, the sensitivity of protoplast-regenerated seedling ofU. prolifera to phosphinothricin and six kinds of antibiotics was tested. Results showed that seedlings were not sensitive to ampicillin, kanamycin, streptomycin sulphate and zeocin, weakly sensitive to chloramphenicol and hygromycin, but very sensitive to phosphinothricin. Moreover, the sensitivity to phosphinothricin was signifi-cantly correlated with photoperiod. The LD50 and 95% confidence range of phosphinothricin were calculated. These results can lay basis for screening transformants and establishing the genetic transformation system ofU. prolifera.