CAJ | 학술논문

分析了酸化环境对马氏珠母贝珍珠层形成的影响。在温度为28℃,盐度为31的条件下,分别在pH 8.2(对照组, CG)、pH 7.7(实验一组, E1)和pH 7.4(实验二组, E2)的海水中养殖马氏珠母贝,观察贝壳珍珠层结晶形貌的变化,并检测珍珠层形成相关的矿化基因(pif177、nacrein及pearlin)在外套膜中央区(mantle center, MC)的表达。结果表明：(1)通过扫描电镜观察贝壳,比较对照组与实验组贝壳晶体形貌变化,发现 E1组贝壳珍珠层低倍镜下,生长阶梯明显,高倍镜下超微结构规则,六边形棱角不明显,但边界清晰；而E2组出现板块边界不明显,生长排列不规则等现象,说明了海水酸化影响了马氏珠母贝的钙沉积,引起珍珠层形貌特征的变化；(2)实验设定条件下,pif177、nacrein及pearlin基因表达量整体上呈现随pH降低而降低的变化趋势。其中, E1组与CG组间均无显著性差异(P>0.05), E2组均较CG组显著下调(P<0.05)。
분석료산화배경대마씨주모패진주층형성적영향。재온도위28℃,염도위31적조건하,분별재pH 8.2(대조조, CG)、pH 7.7(실험일조, E1)화pH 7.4(실험이조, E2)적해수중양식마씨주모패,관찰패각진주층결정형모적변화,병검측진주층형성상관적광화기인(pif177、nacrein급pearlin)재외투막중앙구(mantle center, MC)적표체。결과표명：(1)통과소묘전경관찰패각,비교대조조여실험조패각정체형모변화,발현 E1조패각진주층저배경하,생장계제명현,고배경하초미결구규칙,륙변형릉각불명현,단변계청석；이E2조출현판괴변계불명현,생장배렬불규칙등현상,설명료해수산화영향료마씨주모패적개침적,인기진주층형모특정적변화；(2)실험설정조건하,pif177、nacrein급pearlin기인표체량정체상정현수pH강저이강저적변화추세。기중, E1조여CG조간균무현저성차이(P>0.05), E2조균교CG조현저하조(P<0.05)。
The current study examined the impacts of ocean acidification of on the nacre mineralization process of Pinctada marensii. The experimental pH values of the seawater for breeding experiments were 8.2 (controlling group, CG), 7.7 (experimental group 1, E1) and 7.4 (experimental group 2, E2) with other experimental conditions (28℃, 31‰ salinity) constantly. We observed changes in nacre crystal morphology and examined the expression levels of nacre mineralization associated genes (pif177,nacrein andpearlin) in the mantle center (MC). The results showed that the growth ladder of E1 group nacre was obvious in low magnification, while in high magnification, the ultrastructure was regular. Despite the hexagon edges were not obvious, the border was clear. However, the E2 group had no obvious plate boundary; arranged irregularly, further illustrating the significant impact of seawater acidification on calcification and the nacre crystal morphology ofP. martensii. Under the experimental conditions, the expression ofpif177,nacrein and pearlin, decreased with decrease in pH. Among them, there was no significant difference between the groups E1 and CG group (P>0.05), while the gene expression levels in E2 group were sig-nificantly lower than those in CG group (P<0.05).