CAJ | 학술논문

目的：观察白花蛇舌草总黄酮（FOD）对肝癌 MHCC97- H 细胞增殖和凋亡的影响。方法：将生长良好的细胞分为空白组、FOD 组、5- FU 组及联合组四组，分别干预48小时，观察细胞的形态，MTT 法检测其对肿瘤细胞增殖的抑制作用；流式细胞仪检测 FOD 对 MHCC97- H 肝癌细胞凋亡的影响。结果：FOD 对肝癌MHCC97- H 细胞有显著的抑制作用，并且具有剂量和时间依赖性，不但能使细胞密度减少，细胞变小，核固缩，而且能显著抑制人肝癌 MHCC97- H 细胞的增殖并诱导该细胞的凋亡（P ＜0.05）。结论：白花蛇舌草总黄酮能够通过抑制肿瘤细胞生长、促进肿瘤细胞凋亡，明显抑制肝癌 MHCC97- H 细胞的增殖。
목적：관찰백화사설초총황동（FOD）대간암 MHCC97- H 세포증식화조망적영향。방법：장생장량호적세포분위공백조、FOD 조、5- FU 조급연합조사조，분별간예48소시，관찰세포적형태，MTT 법검측기대종류세포증식적억제작용；류식세포의검측 FOD 대 MHCC97- H 간암세포조망적영향。결과：FOD 대간암MHCC97- H 세포유현저적억제작용，병차구유제량화시간의뢰성，불단능사세포밀도감소，세포변소，핵고축，이차능현저억제인간암 MHCC97- H 세포적증식병유도해세포적조망（P ＜0.05）。결론：백화사설초총황동능구통과억제종류세포생장、촉진종류세포조망，명현억제간암 MHCC97- H 세포적증식。
Objective:To observe the effect of total flavonoids of Oldenlandia diffusa(FOD)on the proliferation and apoptosis of hepatocellular carcinoma MHCC97 - H cells. Methods:MHCC97 - H cells were divided into four groups:control group,FOD group,5 - FU group and combined group,intervented for 48 hours,observe the cells form, detect the inhibitory effect on tumor cell proliferation by MTT method and the influence of FOD on apoptosis of hepa-tocellular carcinoma cell line MHCC97 - H by flow cytometry. Results:FOD had significant inhibitory effect on liver cancer MHCC97 - H cells with dose and time dependence,not only can make the cells density decreased,cells be-come small,nuclear pyknosis,and inhibit the apoptosis of hepatocellular carcinoma MHCC97 - H cells proliferation and induce cell apoptosis(P < 0. 05). Conclusion:Total flavones of Oldenlandia diffusa willa can inhibit the prolifer-ation of hepatocellular carcinoma MHCC97 - H cells by inhibiting the growth of tumor cells and promoting the apopto-sis of tumor cells.