淮北师范大学学报（自然科学版）
淮北師範大學學報（自然科學版）
회북사범대학학보（자연과학판）
Journal of Huaibei Normal University (Natural Science)
2015年
3期
35-40
,共6页
分层修饰电极%多巴胺%循环伏安法%差分脉冲法%L-半胱氨酸
分層脩飾電極%多巴胺%循環伏安法%差分脈遲法%L-半胱氨痠
분층수식전겁%다파알%순배복안법%차분맥충법%L-반광안산
layered modified electrode%dopamine%cyclic voltammetry%differential pulse voltammetry%L-cyste-ine
用循环伏安法制备L-半胱氨酸和银分层修饰电极，用阻抗谱对分层修饰电极进行表征.研究修饰电极上多巴胺的电化学行为，建立差分脉冲法测定痕量多巴胺的新方法.结果表明，在扫描速率为100 mV/s，pH3.0的磷酸盐缓冲溶液中，多巴胺在L-半胱氨酸和银分层修饰电极上产生一对明显的氧化还原峰，峰电位分别为Epa=0.452 V，Epc=0.404 V.用差分脉冲法测定时，峰电流与多巴胺浓度分别在2.50×10-7~7.50×10-6 mol/L和7.50×10-6~1.00×10-4 mol/L呈线性关系，检出限为7.5×10-8 mol/L.用于香蕉中多巴胺的测定，结果满意.
用循環伏安法製備L-半胱氨痠和銀分層脩飾電極，用阻抗譜對分層脩飾電極進行錶徵.研究脩飾電極上多巴胺的電化學行為，建立差分脈遲法測定痕量多巴胺的新方法.結果錶明，在掃描速率為100 mV/s，pH3.0的燐痠鹽緩遲溶液中，多巴胺在L-半胱氨痠和銀分層脩飾電極上產生一對明顯的氧化還原峰，峰電位分彆為Epa=0.452 V，Epc=0.404 V.用差分脈遲法測定時，峰電流與多巴胺濃度分彆在2.50×10-7~7.50×10-6 mol/L和7.50×10-6~1.00×10-4 mol/L呈線性關繫，檢齣限為7.5×10-8 mol/L.用于香蕉中多巴胺的測定，結果滿意.
용순배복안법제비L-반광안산화은분층수식전겁，용조항보대분층수식전겁진행표정.연구수식전겁상다파알적전화학행위，건립차분맥충법측정흔량다파알적신방법.결과표명，재소묘속솔위100 mV/s，pH3.0적린산염완충용액중，다파알재L-반광안산화은분층수식전겁상산생일대명현적양화환원봉，봉전위분별위Epa=0.452 V，Epc=0.404 V.용차분맥충법측정시，봉전류여다파알농도분별재2.50×10-7~7.50×10-6 mol/L화7.50×10-6~1.00×10-4 mol/L정선성관계，검출한위7.5×10-8 mol/L.용우향초중다파알적측정，결과만의.
Silver and L-cysteine layered modified electrode was fabricated by cyclic voltammetry. The modi?fied electrode was characterized by impedance spectrum. The electrochemical behavior of dopamine was stud?ied by cyclic voltammetry on the modified electrode. A new method was developed for the determination of trace dopamine. The experimental results indicated that in phosphate buffer solution at pH3.0 with the scan rate of 100 mV/s,a pair of redox peaks were observed at peak potential Epa=0.452 V and Epc=0.404 V. The linear ranges for the determination dopamine were 2.50×10-7~7.50×10-6 mol/L and 7.50×10-6~1.00×10-4 mol/L. The detection limit was 7.5×10-8 mol/L. The method has been applied to the determination of dopamine in the banana with satisfactory results.
