现代农业科技
現代農業科技
현대농업과기
XianDai NongYe KeJi
2015年
16期
125-126,131
,共3页
气相色谱法%豇豆%土壤%烯啶虫胺
氣相色譜法%豇豆%土壤%烯啶蟲胺
기상색보법%강두%토양%희정충알
gas chromatography(GC)%cowpeas%soil%nitenpyram
建立了豇豆和土壤样品中烯啶虫胺残留量的快速检测方法，样品用乙腈-水（4：1，v/v），振荡提取3h，浓缩后甲醇定容，气相色谱-电子捕获检测器（GC-ECD）检测，外标法定量。结果表明：乙腈提取豇豆和土壤中烯啶虫胺效果较好，无需净化可以直接上机检测。该方法在烯啶虫胺添加量为0.01~1.00 mg/L范围内线性相关性良好，R2=0.9991，最低检出限为0.005 mg/kg。在添加水平为0.01~1.00 mg/kg中，豇豆样品在3个烯啶虫胺添加水平下的回收率为86.8%~101.5%，相对标准偏差为3.3%~4.9%；土壤样品在3个烯啶虫胺添加水平下的回收率为83.8%~105.1%，相对标准偏差为5.8%~8.9%，其具有高效率、低成本、高灵敏度、定量准确等优点。
建立瞭豇豆和土壤樣品中烯啶蟲胺殘留量的快速檢測方法，樣品用乙腈-水（4：1，v/v），振盪提取3h，濃縮後甲醇定容，氣相色譜-電子捕穫檢測器（GC-ECD）檢測，外標法定量。結果錶明：乙腈提取豇豆和土壤中烯啶蟲胺效果較好，無需淨化可以直接上機檢測。該方法在烯啶蟲胺添加量為0.01~1.00 mg/L範圍內線性相關性良好，R2=0.9991，最低檢齣限為0.005 mg/kg。在添加水平為0.01~1.00 mg/kg中，豇豆樣品在3箇烯啶蟲胺添加水平下的迴收率為86.8%~101.5%，相對標準偏差為3.3%~4.9%；土壤樣品在3箇烯啶蟲胺添加水平下的迴收率為83.8%~105.1%，相對標準偏差為5.8%~8.9%，其具有高效率、低成本、高靈敏度、定量準確等優點。
건립료강두화토양양품중희정충알잔류량적쾌속검측방법，양품용을정-수（4：1，v/v），진탕제취3h，농축후갑순정용，기상색보-전자포획검측기（GC-ECD）검측，외표법정량。결과표명：을정제취강두화토양중희정충알효과교호，무수정화가이직접상궤검측。해방법재희정충알첨가량위0.01~1.00 mg/L범위내선성상관성량호，R2=0.9991，최저검출한위0.005 mg/kg。재첨가수평위0.01~1.00 mg/kg중，강두양품재3개희정충알첨가수평하적회수솔위86.8%~101.5%，상대표준편차위3.3%~4.9%；토양양품재3개희정충알첨가수평하적회수솔위83.8%~105.1%，상대표준편차위5.8%~8.9%，기구유고효솔、저성본、고령민도、정량준학등우점。
A rapid analytical method was developed for the determination of nitenpyram residue in cowpeas and soil by gas chromatography(GC). The nitenpyram was extracted from cowpeas and soil with acetonitrile-water(4:1,v/v)in a separatory funnel,and oscillation and extracting for 3 hours,then determined by GC-ECD with external standard method. The results showed that acetonitrile used as extract solvent was favorable for the extraction of nitenpyram without purification.A good linearity ranged from 0.01-1.00 mg/L was obtained and the correlation coefficient was 0.999 1.The limit of detection(LOD)was 0.005 mg/kg.The recovery of nitenpyram in cowpeas and soil was 86.8%-101.5%and 83.8%-105.1%,respectively at three spiked levels ranged from 0.01-1.00 mg/kg.The corresponding relation standard deviation(RSD)was 3.3%-4.9% and 5.8%-8.9%,respectively. Compared with the other methods,this method had the advantages of high efficiency,low cost,high sensitivity,accurate etc..
