CAJ | 학술논문

为了解猪流行性腹泻（PED ）在福建省的流行和流行毒株S基因的变异情况，对2011－2014年采自117个规模猪场的295份疑似PED的病料进行检测，对14株具有代表性的PEDV毒株进行S基因序列分析。结果显示，2011－2014年病料阳性率分别为87.30％、75.00％、40.91％和34.15％，总阳性率为69.49％，虽然PED阳性率在福建省有下降趋势，但仍较高。基因序列分析表明福建省14株PEDV分离株S基因核苷酸之间的同源性分别为98.5％～100.0％，与国内其他毒株之间的同源性为93.3％～99.5％，其中与国内2009年以前流行的PEDV毒株的同源性较低；与2008年泰国 NPPED2008＿2株的同源性分别为95.8％～96.1％；与CV777标准株的同源性为93.8％～94.1％。分离毒株的S基因片段氨基酸存在多个位点突变、插入和缺失现象。遗传进化树表明14株 PEDV 分离株与2008年泰国 NPPED2008＿2毒株和2009年韩国毒株亲缘关系较近，与attenuated DR13弱毒株及CV777标准株亲缘关系比较远。14株PEDV的S基因序列已登陆Genbank。
위료해저류행성복사（PED ）재복건성적류행화류행독주S기인적변이정황，대2011－2014년채자117개규모저장적295빈의사PED적병료진행검측，대14주구유대표성적PEDV독주진행S기인서렬분석。결과현시，2011－2014년병료양성솔분별위87.30％、75.00％、40.91％화34.15％，총양성솔위69.49％，수연PED양성솔재복건성유하강추세，단잉교고。기인서렬분석표명복건성14주PEDV분리주S기인핵감산지간적동원성분별위98.5％～100.0％，여국내기타독주지간적동원성위93.3％～99.5％，기중여국내2009년이전류행적PEDV독주적동원성교저；여2008년태국 NPPED2008＿2주적동원성분별위95.8％～96.1％；여CV777표준주적동원성위93.8％～94.1％。분리독주적S기인편단안기산존재다개위점돌변、삽입화결실현상。유전진화수표명14주 PEDV 분리주여2008년태국 NPPED2008＿2독주화2009년한국독주친연관계교근，여attenuated DR13약독주급CV777표준주친연관계비교원。14주PEDV적S기인서렬이등륙Genbank。
The intensity and widespread of the porcine epidemic diarrhea (PED) have continuously been on the climb in Fujian since the end of 2010. The infectious disease caused high mortalities in suckling piglets ,and has become one of the most serious concerns for the pig farms in the area .To deter the epidemic and understand the genetic variations of S genes of PED viruses ,295 clinical samples from 117 sizable pig farms were collected during 2011 -2014 for RT‐PCR analysis .Overall ,205 out of 295 samples (69.49% ) were tested positive .Annually ,the infection rate declined from 87.3% ,75.00% ,40.91% to 34.15% during the year of 2011 to 2014. Nonetheless ,it remained a serious problem for the industry .The study on nucleotide sequence revealed that the S genes of the 14 isolated PED strains shared 98.5% -100.0% homogeneity among themselves ,and 93.3% -99.5% in comparison with other strains in China ,93.8% -94.1% with CV777 ,and 95.8% - 96.1% with NPPED2008 _ 2. Variants , insertions and deletions of amino acids on the genes were found .The phylogenetic analysis on the 14 PED viruses showed a close relationship with the Thailand and Korean strains ,and a remote one with the attenuated DR13 and CV777. The S gene sequence obtained from this study has been filed with Genbank .