听力学及言语疾病杂志
聽力學及言語疾病雜誌
은역학급언어질병잡지
Journal of Audiology and Speech Pathology
2015年
5期
510-514
,共5页
快速老化小鼠%听性脑干反应%耳蜗%磷酸化的细胞外信号调节激酶1/2
快速老化小鼠%聽性腦榦反應%耳蝸%燐痠化的細胞外信號調節激酶1/2
쾌속노화소서%은성뇌간반응%이와%린산화적세포외신호조절격매1/2
Senescence accelerated mouse%Auditory brainstem response%Cochlea%P-ERK1/2
目的：探讨快速老化小鼠的听功能及耳蜗组织中磷酸化的细胞外信号调节激酶1／2（phosphorylated extracellular signal －regulated protein kinases ，p－ERK1／2）表达的增龄性变化。方法选用3、5、7月龄的快速老化小鼠亚系8（SAMP8）各6只，分别进行8 kHz短纯音听性脑干反应（ABR）检测，并用免疫组化染色方法分别检测各月龄组小鼠耳蜗组织p－ERK1／2的表达，分析其増龄性变化。结果3、5、7月龄SAM P8小鼠ABR阈值左耳分别为31．817±1．228、54．329±1．459、58．330±1．252 dB S PL ，右耳分别为32．474±1．041、53．485±1．385、57．842±1．173 dB SPL ，SAMP8小鼠双耳听性脑干反应阈值随月龄增加而明显提高（P＜0．05）；3月龄小鼠螺旋神经节细胞、毛细胞中p－EPK1／2平均光密度值分别为0．6997±0．0188、0．6519±0．0252；5月龄小鼠螺旋神经节细胞、毛细胞中p－EPK1／2平均光密度值分别为0．6215±0．0147、0．5912±0．0102；7月龄小鼠螺旋神经节细胞、毛细胞中p－EPK1／2平均光密度值分别为0．5753±0．0155、0．5593±0．0067，p－EPK1／2在不同月龄快速老化小鼠耳蜗组织中的表达光密度值随着月龄增加而显著降低（P＜0．05）。结论快速老化小鼠耳蜗组织中p－EPK1／2蛋白的表达水平随着年龄相关性听功能减退而降低，推测p－EPK1／2可能与维持耳蜗功能状态及听觉形成有关。
目的：探討快速老化小鼠的聽功能及耳蝸組織中燐痠化的細胞外信號調節激酶1／2（phosphorylated extracellular signal －regulated protein kinases ，p－ERK1／2）錶達的增齡性變化。方法選用3、5、7月齡的快速老化小鼠亞繫8（SAMP8）各6隻，分彆進行8 kHz短純音聽性腦榦反應（ABR）檢測，併用免疫組化染色方法分彆檢測各月齡組小鼠耳蝸組織p－ERK1／2的錶達，分析其増齡性變化。結果3、5、7月齡SAM P8小鼠ABR閾值左耳分彆為31．817±1．228、54．329±1．459、58．330±1．252 dB S PL ，右耳分彆為32．474±1．041、53．485±1．385、57．842±1．173 dB SPL ，SAMP8小鼠雙耳聽性腦榦反應閾值隨月齡增加而明顯提高（P＜0．05）；3月齡小鼠螺鏇神經節細胞、毛細胞中p－EPK1／2平均光密度值分彆為0．6997±0．0188、0．6519±0．0252；5月齡小鼠螺鏇神經節細胞、毛細胞中p－EPK1／2平均光密度值分彆為0．6215±0．0147、0．5912±0．0102；7月齡小鼠螺鏇神經節細胞、毛細胞中p－EPK1／2平均光密度值分彆為0．5753±0．0155、0．5593±0．0067，p－EPK1／2在不同月齡快速老化小鼠耳蝸組織中的錶達光密度值隨著月齡增加而顯著降低（P＜0．05）。結論快速老化小鼠耳蝸組織中p－EPK1／2蛋白的錶達水平隨著年齡相關性聽功能減退而降低，推測p－EPK1／2可能與維持耳蝸功能狀態及聽覺形成有關。
목적：탐토쾌속노화소서적은공능급이와조직중린산화적세포외신호조절격매1／2（phosphorylated extracellular signal －regulated protein kinases ，p－ERK1／2）표체적증령성변화。방법선용3、5、7월령적쾌속노화소서아계8（SAMP8）각6지，분별진행8 kHz단순음은성뇌간반응（ABR）검측，병용면역조화염색방법분별검측각월령조소서이와조직p－ERK1／2적표체，분석기증령성변화。결과3、5、7월령SAM P8소서ABR역치좌이분별위31．817±1．228、54．329±1．459、58．330±1．252 dB S PL ，우이분별위32．474±1．041、53．485±1．385、57．842±1．173 dB SPL ，SAMP8소서쌍이은성뇌간반응역치수월령증가이명현제고（P＜0．05）；3월령소서라선신경절세포、모세포중p－EPK1／2평균광밀도치분별위0．6997±0．0188、0．6519±0．0252；5월령소서라선신경절세포、모세포중p－EPK1／2평균광밀도치분별위0．6215±0．0147、0．5912±0．0102；7월령소서라선신경절세포、모세포중p－EPK1／2평균광밀도치분별위0．5753±0．0155、0．5593±0．0067，p－EPK1／2재불동월령쾌속노화소서이와조직중적표체광밀도치수착월령증가이현저강저（P＜0．05）。결론쾌속노화소서이와조직중p－EPK1／2단백적표체수평수착년령상관성은공능감퇴이강저，추측p－EPK1／2가능여유지이와공능상태급은각형성유관。
Objective To investigate degenerative changes of auditory functions and age -related expression of phosphorylated extracellular signal -regulated protein kinases1/2(p-ERK1/2) in the cochlea of the senescence accelerated mouse .Methods The 3 ,5 ,7 months old mice were selected for the study and each group had 6 mice . The 8 kHz tone burst auditory thresholds and age -related expression of p -ERK1/2 in the cochlea were studied in the senescence accelerated mouse/prone 8(SAMP8) at 3 ,5 ,7 months .The expression of p -ERK1/2 was analyzed by the optical density of immunohistochemical staining .Results For the auditory function evaluation :The SAMP 8 developed a progressive hearing loss at 8 kHz which showed an age -related significant increase (P< 0 .05) .The ABR thresholds in 3 ,5 ,7 months old groups in the left ear were 31 .817 ± 1 .228 ,54 .329 ± 1 .459 ,58 .330 ± 1 .252 dB SPL ,respectively .In the right ears ,the ABR threshold were 32 .474 ± 1 .041 ,53 .485 ± 1 .385 ,57 .842 ± 1 .173 dB SPL ,respectively .p-ERK1/2 protein expressed in the cochlea of the SAMP8 throughout the development sta‐ges ,which developed an age-related significant decrease (P<0 .05) .The average optical density of p -ERK1/2 in the spiral ganglion cells in the 3 ,5 ,7 months old mice were 0 .699 7 ± 0 .018 8 ,0 .621 5 ± 0 .014 7 ,0 .575 3 ± 0 .015 5 ,respectively .In the hair cells ,they w ere 0 .651 9 ± 0 .025 2 ,0 .591 2 ± 0 .010 2 ,0 .559 3 ± 0 .006 7 respec‐tively .Conclusion The expression level of p -ERK1/2 protein decreases when the SAMP 8 develops a progressive hearing loss .This indicates that p-ERK1/2 protein probably has relationship with maintaining functional status of the cochlea and the auditory formation .
