CAJ | 학술논문

目的：分析喉癌肿瘤组织中端粒酶逆转录酶（telomerase reverse transcriptase ，T ERT ）与转录激活蛋白－1（acticator protein 1，AP－1）基因 mRNA和蛋白表达水平及两者的相关性。方法利用RT －PCR及量子点标记的免疫荧光染色法，检测24例人喉癌组织中TERT和AP－1的表达。结果在喉癌组织中，TERT与AP－1基因mRNA及蛋白表达水平呈显著正相关，相关系数分别为0．574和0．809，有显著统计学意义（P＜0．01）。结论 TERT与AP－1在喉癌组织中的表达具有高度一致性。
목적：분석후암종류조직중단립매역전록매（telomerase reverse transcriptase ，T ERT ）여전록격활단백－1（acticator protein 1，AP－1）기인 mRNA화단백표체수평급량자적상관성。방법이용RT －PCR급양자점표기적면역형광염색법，검측24례인후암조직중TERT화AP－1적표체。결과재후암조직중，TERT여AP－1기인mRNA급단백표체수평정현저정상관，상관계수분별위0．574화0．809，유현저통계학의의（P＜0．01）。결론 TERT여AP－1재후암조직중적표체구유고도일치성。
Objective To investigate telomerase reverse transcriptase (TERT ) and acticator protein 1(AP -1) expression and it's corroloation in laryngeal carcinoma tissue .Methods 24 human laryngeal carcinoma tissue were analised by RT -PCR and quantum -dot based immunofluorescence assay for the expression of TERT and AP -1 . Results The expression of TERT mRNA and the expression of c -Fos ,c-Jun in laryngeal carcinoma were postive‐ly related .Correlation coefficient was 0 .574 and 0 .809 ,respectivly(P<0 .01) .Conclusion TERT and AP -1 were expressed at high levels and positively correlated in human laryngeal carcinoma .