CAJ | 학술논문

目的：分析葡萄膜恶性黑色素瘤患者血管内皮细胞生长因子( vascular endothelial growth factor,VEGF)的表达水平是否与葡萄膜恶性黑色素瘤的转移和预后有关。 　　方法：采用ELISA法检测葡萄膜恶性黑色素瘤患者和正常对照组中外周血VEGF水平,并采用RT-PCR和Western-blot检测葡萄膜恶性黑色素瘤患者肿瘤组织中 VEGF mRNA和VEGF蛋白表达水平。对葡萄膜恶性黑色素瘤患者进行随访,用Kaplan-Meier法分析基线VEGF水平与患者术后转移的关系。 　　结果：葡萄膜恶性黑色素瘤患者外周血VEGF水平较健康者升高。就诊时肿瘤发生转移的患者 VEGF mRNA 和VEGF蛋白表达水平均较未发生转移的患者升高。在随访过程中,血清VEGF≥700 pg/mL的患者,肿瘤发生转移的风险较高。 　　结论：葡萄膜恶性黑色素瘤患者中VEGF的表达水平与葡萄膜恶性黑色素瘤的转移密切相关。
목적：분석포도막악성흑색소류환자혈관내피세포생장인자( vascular endothelial growth factor,VEGF)적표체수평시부여포도막악성흑색소류적전이화예후유관。 　　방법：채용ELISA법검측포도막악성흑색소류환자화정상대조조중외주혈VEGF수평,병채용RT-PCR화Western-blot검측포도막악성흑색소류환자종류조직중 VEGF mRNA화VEGF단백표체수평。대포도막악성흑색소류환자진행수방,용Kaplan-Meier법분석기선VEGF수평여환자술후전이적관계。 　　결과：포도막악성흑색소류환자외주혈VEGF수평교건강자승고。취진시종류발생전이적환자 VEGF mRNA 화VEGF단백표체수평균교미발생전이적환자승고。재수방과정중,혈청VEGF≥700 pg/mL적환자,종류발생전이적풍험교고。 　　결론：포도막악성흑색소류환자중VEGF적표체수평여포도막악성흑색소류적전이밀절상관。
AIM:To identify relationship between the expression of vascular endothelial growth factor ( VEGF ) of uveal melanoma patients whether with uveal melanoma metastasis and prognosis. METHODS: The peripheral blood VEGF of uveal melanoma patients and patients in normal control group were detected by enzyme-linked immunosorbent assay ( ELISA ) . The mRNA and protein expression level of VEGF in uveal melanoma tissues were analyzed through reverse transcription- polymerase chain reaction ( RT-PCR) and Western-blot. Uveal melanoma patients were followed up, and the relationship of baseline serum VEGF and postoperative metastasis was analyzed by Kaplan-Meier method. RESULTS: The expression level of peripheral blood VEGF was significantly increased in uveal melanoma patients than that in healthy people. The mRNA and protein expression level of VEGF in the uveal melanoma patients with metastasis when seeing a doctor were higher than patients without metastasis. In the process of follow-up, the uveal melanoma patients ( serum VEGF ≥700pg/mL) had higher risk for tumor metastasis. CONCLUSION: The expression level of VEGF of uveal melanoma patients is closely related to uveal melanoma metastasis.