肉类研究
肉類研究
육류연구
Meat Research
2015年
8期
28-30
,共3页
郑卫%徐世明%赵瑞连%张建梅%郭光平%孙承锋%王光杰
鄭衛%徐世明%趙瑞連%張建梅%郭光平%孫承鋒%王光傑
정위%서세명%조서련%장건매%곽광평%손승봉%왕광걸
油炸肉制品%硅胶%固相萃取%高效液相色谱
油炸肉製品%硅膠%固相萃取%高效液相色譜
유작육제품%규효%고상췌취%고효액상색보
fried meat products%silica gel%solid phase extraction%high performance liquid chromatography (HPLC)
实验建立一种利用硅胶固相萃取-高效液相色谱来检测油炸肉制品中丙烯酰胺的含量的方法。采用硅胶作为萃取材料对样品提取液进行净化，利用高效液相色谱进行检测。结果表明：检测波长为205 nm，流动相为甲醇-水（5∶95，V/V），流速为0.8 mL/min，方法加标回收率为73.5%～80.8%，相对标准偏差≤5.54%，最低检出限为10 ng/mL。该方法简便、可靠，满足油炸肉制品中丙烯酰胺的检测要求。
實驗建立一種利用硅膠固相萃取-高效液相色譜來檢測油炸肉製品中丙烯酰胺的含量的方法。採用硅膠作為萃取材料對樣品提取液進行淨化，利用高效液相色譜進行檢測。結果錶明：檢測波長為205 nm，流動相為甲醇-水（5∶95，V/V），流速為0.8 mL/min，方法加標迴收率為73.5%～80.8%，相對標準偏差≤5.54%，最低檢齣限為10 ng/mL。該方法簡便、可靠，滿足油炸肉製品中丙烯酰胺的檢測要求。
실험건립일충이용규효고상췌취-고효액상색보래검측유작육제품중병희선알적함량적방법。채용규효작위췌취재료대양품제취액진행정화，이용고효액상색보진행검측。결과표명：검측파장위205 nm，류동상위갑순-수（5∶95，V/V），류속위0.8 mL/min，방법가표회수솔위73.5%～80.8%，상대표준편차≤5.54%，최저검출한위10 ng/mL。해방법간편、가고，만족유작육제품중병희선알적검측요구。
This paper presents a silica gel solid phase extraction-high performance liquid chromatography (HPLC) method to detect the content of acrylamide in fried meat products. The extract was cleaned up by solid phase extraction using silica gel as the adsorbent prior to being analyzed by HPLC. The detection wavelength was set at 205 nm. The separation was performed using a Kromisil C18 (250 mm × 4.6 mm, 5 μm). The mobile phase used was a 5:95 (V/V)mixture of methanol and water at a lfow rate of 0.8 mL/min. Recoveries of the proposed method for spiked samples ranged from 73.5% to 80.8%, with relative standard deviation (RSD) no greater than 5.54% (n= 5). The limit of detection was 10 ng/mL (RS/N= 3). This method is simple and reliable, and can be used for the detection of acrylamide in fried meat products.
