肉类研究
肉類研究
육류연구
Meat Research
2015年
8期
17-21
,共5页
山梨糖醇%肉制品%高效液相色谱-蒸发光散射法
山梨糖醇%肉製品%高效液相色譜-蒸髮光散射法
산리당순%육제품%고효액상색보-증발광산사법
sorbitol%meat products%high performance liquid chromatography-evaporation light scattering detector (HPLC-ELSD)
建立肉制品中山梨糖醇的高效液相色谱-蒸发光散射检测器（high performance liquid chromatography-evaporation light scattering detector，HPLC-ELSD）测定法。采用乙腈-水溶液（7∶3，V/V）作为流动相直接超声提取样品，经Agilent ZORBAX NH2（4.6 mm×250 mm，5μm）色谱柱分离，蒸发光散射检测器检测；优化ELSD检测器参数为飘移管温度90℃，氮气流速2.0 L/min。结果表明：山梨糖醇含量在0.2～5.0 mg/mL范围内有良好的线性，方法相对标准偏差（relative standard deviation，RSD）在1.28%～2.02%之间，回收率在88.3%～99.0%之间。本方法具有样品前处理简便、方法检出限低、方法重现性好的特点，适用于肉制品中山梨糖醇含量的测定。
建立肉製品中山梨糖醇的高效液相色譜-蒸髮光散射檢測器（high performance liquid chromatography-evaporation light scattering detector，HPLC-ELSD）測定法。採用乙腈-水溶液（7∶3，V/V）作為流動相直接超聲提取樣品，經Agilent ZORBAX NH2（4.6 mm×250 mm，5μm）色譜柱分離，蒸髮光散射檢測器檢測；優化ELSD檢測器參數為飄移管溫度90℃，氮氣流速2.0 L/min。結果錶明：山梨糖醇含量在0.2～5.0 mg/mL範圍內有良好的線性，方法相對標準偏差（relative standard deviation，RSD）在1.28%～2.02%之間，迴收率在88.3%～99.0%之間。本方法具有樣品前處理簡便、方法檢齣限低、方法重現性好的特點，適用于肉製品中山梨糖醇含量的測定。
건립육제품중산리당순적고효액상색보-증발광산사검측기（high performance liquid chromatography-evaporation light scattering detector，HPLC-ELSD）측정법。채용을정-수용액（7∶3，V/V）작위류동상직접초성제취양품，경Agilent ZORBAX NH2（4.6 mm×250 mm，5μm）색보주분리，증발광산사검측기검측；우화ELSD검측기삼수위표이관온도90℃，담기류속2.0 L/min。결과표명：산리당순함량재0.2～5.0 mg/mL범위내유량호적선성，방법상대표준편차（relative standard deviation，RSD）재1.28%～2.02%지간，회수솔재88.3%～99.0%지간。본방법구유양품전처리간편、방법검출한저、방법중현성호적특점，괄용우육제품중산리당순함량적측정。
A high performance liquid chromatography with evaporative light-scattering detection (HPLC-ELSD) method was developed for the determination of sorbitol in meat products .The samples were ultrasonically extracted using acetonitrile water (7:3,V/V) as mobile phase and separated on ZORBAX NH2 Agilent column, then detected by evaporative light scattering detector (ELSD). Optimized ELSD detector parameters such as drift tube temperature and nitrogen flow rate were set at 90℃ and 2.0 L/min, respectively. The results showed that sorbitol displayed good linearity relationship over the concentration range of 0.2 to 10 mg/mL. The method precision (relative standard deviation, RSD) ranged from 1.28% to 2.02%, and the recovery was between 88.3% and 99.0%. This method has the advantages of simple sample pretreatment, low detection limit and good reproducibility. So it is useful for the determination of sorbitol in meat products.
