CAJ | 학술논문

CRISPR/Cas9（Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-associated nuclease 9）基因编辑系统是基于古细菌抵御外源核酸入侵的免疫机制为基础开发出来的一种新型的基因编辑技术。相对于传统的基因编辑系统，该系统具有更加高效、操作简单、细胞毒性小等特点。目前，CRISPR/Cas9基因编辑技术已经在肿瘤研究的诸多方面中得到应用，包括肿瘤相关基因的功能研究、构建动物肿瘤模型、筛选肿瘤细胞表型及耐药相关基因以及肿瘤的基因治疗等诸多方面。本文就其在肿瘤研究中的应用进展进行综述。
CRISPR/Cas9（Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-associated nuclease 9）기인편집계통시기우고세균저어외원핵산입침적면역궤제위기출개발출래적일충신형적기인편집기술。상대우전통적기인편집계통，해계통구유경가고효、조작간단、세포독성소등특점。목전，CRISPR/Cas9기인편집기술이경재종류연구적제다방면중득도응용，포괄종류상관기인적공능연구、구건동물종류모형、사선종류세포표형급내약상관기인이급종류적기인치료등제다방면。본문취기재종류연구중적응용진전진행종술。
TCRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-associated nuclease 9) gene editing system is a new type of gene editing technology developed based on the immune mechanism of ar-chaea resisting the invasion of exogenous nucleic acid. Compared with traditional gene editing system, CRISPR/Cas9 system is more effcient, easier operating, and less cytotoxic. Currently, CRISPR/Cas9 gene editing technology has been applied to many aspects of cancer research, including research on cancer genes, constructing animal tumor models, screening tumor resistance-associated and phenotypic-related genes and cancer gene therapy. In this review, the application of the CRISPR/Cas9 system in tumor research were introduced.