CAJ | 학술논문

筛选富集纯化毛脉酸模乙酸乙酯部位（白藜芦醇、白藜芦醇苷、大黄素、芦荟大黄素、大黄酚、大黄酚苷）成分的最佳树脂，优化大孔树脂纯化目标成分的最佳工艺．以白藜芦醇、白藜芦醇苷、大黄素、芦荟大黄素、大黄酚、大黄酚苷的吸附率和解吸率为评价指标，筛选树脂，并优化了吸附和洗脱条件．D101树脂对毛脉酸模乙酸乙酯部位成分具有较好的吸附分离性能．最佳工艺条件为，毛脉酸模乙酸乙酯部位样品溶液1 BV，吸附流速为2 BV· h－1，先用2 BV水洗涤，再用20％、50％、75％、95％乙醇各2．5、5、2．5、5 BV进行梯度洗脱，流速2 BV· h－1，合并50％和95％乙醇洗脱液，即为纯化部位．纯化后目标成分纯度提高到49．85％，说明采用D101树脂分离纯化毛脉酸模乙酸乙酯部位成分是可行的．
사선부집순화모맥산모을산을지부위（백려호순、백려호순감、대황소、호회대황소、대황분、대황분감）성분적최가수지，우화대공수지순화목표성분적최가공예．이백려호순、백려호순감、대황소、호회대황소、대황분、대황분감적흡부솔화해흡솔위평개지표，사선수지，병우화료흡부화세탈조건．D101수지대모맥산모을산을지부위성분구유교호적흡부분리성능．최가공예조건위，모맥산모을산을지부위양품용액1 BV，흡부류속위2 BV· h－1，선용2 BV수세조，재용20％、50％、75％、95％을순각2．5、5、2．5、5 BV진행제도세탈，류속2 BV· h－1，합병50％화95％을순세탈액，즉위순화부위．순화후목표성분순도제고도49．85％，설명채용D101수지분리순화모맥산모을산을지부위성분시가행적．
To choose the optimum macroporous resin for enrichment and purification of ethyl acetate extract from Rumex gmelini Turcz , the adsorption rate and desorption rate of resvera-trol, polydatin, rheum emodin, aloe-emodin, chrysophanol and chrysophamol monoglyco-side were used as evaluation indicators .The adsorption and elution conditions were optimized at the same time.As a result, D101 macroporous resin was chosed for its better performance of adsorption and separation and the best process conditions were screened out .The sample solution of ethyl acetate was 1 BV, adsorption flow rate was 2BV· h-1 , after washed by 2BV water, using 2.5BV 20%alcohol, 5BV 50%alcohol, 2.5BV 75% alcohol and 5BV 95%alcohol to perform the gradient elute in due order with the flow rate of 2 BV· h-1 .The elute of 50%alcohol and 95%alcohol were combined to get the depuration that the purity of target compositions were raised to 49.85%.This paper illustrated that the D101 macroporous resin was viable for purification ethyl acetate extract from Rumex gmelini Turcz .