CAJ | 학술논문

目的：探讨橙皮苷对大鼠心肌缺血／再灌注损伤诱导的心肌细胞自噬的影响及其可能机制。方法24只雄性SD大鼠随机均分为假手术组、缺血／再灌注（ I／R）组及橙皮苷组，每组8只。假手术组予0？．5％羧基纤维素钠灌胃3 d后，开胸暴露冠状动脉左前降支（ LAD），并不结扎，I／R组及橙皮苷组予以阻断LAD血流30 min后再灌注4 h，建立大鼠心肌缺血／再灌注损伤模型。术后，检测大鼠血清中肌酸激酶（ CK）和乳酸脱氢酶（ LDH），心肌组织中超氧化物歧化酶（ SOD）、丙二醛（ MDA）及自噬蛋白微管相关蛋白1轻链3Ⅱ（ LC3Ⅱ）和Beclin1的水平。结果与假手术组相比，I／R组大鼠血清中CK及LDH水平均显著升高，心肌组织SOD活性显著降低，MDA含量显著升高（ P均＜0．05）；与I／R组比较，橙皮苷组大鼠血清中CK及LDH水平明显降低，心肌组织SOD活性显著升高，MDA含量明显降低（ P均＜0．05）。与假手术组比较，I／R组大鼠心肌组织LC3Ⅱ及Beclin1表达水平显著增高，与I／R组比较，橙皮苷组上述2项指标表达水平明显降低（ P ＜0．05）。结论橙皮苷可抑制心肌缺血／再灌注损伤诱导的心肌细胞自噬，其机制与橙皮苷减轻氧化应激相关。
목적：탐토등피감대대서심기결혈／재관주손상유도적심기세포자서적영향급기가능궤제。방법24지웅성SD대서수궤균분위가수술조、결혈／재관주（ I／R）조급등피감조，매조8지。가수술조여0？．5％최기섬유소납관위3 d후，개흉폭로관상동맥좌전강지（ LAD），병불결찰，I／R조급등피감조여이조단LAD혈류30 min후재관주4 h，건립대서심기결혈／재관주손상모형。술후，검측대서혈청중기산격매（ CK）화유산탈경매（ LDH），심기조직중초양화물기화매（ SOD）、병이철（ MDA）급자서단백미관상관단백1경련3Ⅱ（ LC3Ⅱ）화Beclin1적수평。결과여가수술조상비，I／R조대서혈청중CK급LDH수평균현저승고，심기조직SOD활성현저강저，MDA함량현저승고（ P균＜0．05）；여I／R조비교，등피감조대서혈청중CK급LDH수평명현강저，심기조직SOD활성현저승고，MDA함량명현강저（ P균＜0．05）。여가수술조비교，I／R조대서심기조직LC3Ⅱ급Beclin1표체수평현저증고，여I／R조비교，등피감조상술2항지표표체수평명현강저（ P ＜0．05）。결론등피감가억제심기결혈／재관주손상유도적심기세포자서，기궤제여등피감감경양화응격상관。
Objective To investigate the effect of hesperidin on myocardial ischemia /reperfusion injury of myocardial cells induced by autophagy and its possible mechanism .Methods 24 male SD rats were randomly divided into sham operation group, ischemia /reperfusion (I/R) group and hesperidin group with 8 rats in each group.Sham operation group were treated with 0.5% carboxymethylcellulose sodium orally , after 3 days, thoracotomy were performed to expose the left anterior de-scending coronary artery ( LAD) but not ligation , in I/R group and hesperidin group were blocking LAD ’ s flow for 30 min, then with 4 hours of reperfusion to establish the rat myocardial ischemia /reperfusion injury model .After the operation , detec-ted the serum creatine kinase (CK) and lactate dehydrogenase (LDH), superoxide dismutase (SOD) and malondialdehyde ( MDA) and autophagy protein , microtubule associated protein 1 light chain 3 II ( LC3 II) and Beclin1 level in the myocardial tissue.Results Compared to sham operation group , I/R group rats’ serum CK and LDH levels were significantly increased , SOD activity of myocardial tissue were significantly decreased , MDA content significantly increased ( P <0 .05 ) , compared with I/R group, hesperidin group’s serum CK and LDH levels significantly decreased , SOD activity of myocardial tissue was significantly increased, MDA content decreased significantly ( P <0.05).Compared with the sham group, I/R group rats’ myocardial tissue LC3 II and Beclin1 levels increased significantly , compared with I/R group, hesperidin group ’ s index ex-pression levels were significantly lower ( P <0.05).Conclusion Hesperidin can inhibit myocardial ischemia /reperfusion injury induced cardiomyocyte autophagy , and the mechanism is related with hesperidin reduce the oxidative stress .