福建农业学报
福建農業學報
복건농업학보
Fujian Journal of Agricultural Sciences
2015年
7期
705-708
,共4页
大花蕙兰疫病%棕榈疫霉%生长特性
大花蕙蘭疫病%棕櫚疫黴%生長特性
대화혜란역병%종려역매%생장특성
Cymbidium blight%Phytophthora palmivora%growth characteristics
对引起福建地区大花蕙兰疫病的棕榈疫霉菌进行生长特性的研究。结果表明:不同培养基对该菌生长影响不同,菌丝在V8汁培养基上生长最快,在清水琼脂培养基上生长最慢,不同培养基上菌落平均直径依次是:V8汁>大花蕙兰叶煎汁﹥番茄汁> PDA= PSA>玉米粉>清水琼脂,最大与最小菌落平均直径差为3.23 cm ,且菌落形态有差异;碳源和氮源以甘露醇和牛肉膏最佳;病原菌在15~35℃, pH3~10的条件下均能生长,最适的生长温度为30℃,最适的p H值为6;菌丝的致死温度为50℃;光暗交替有利于该菌的生长。
對引起福建地區大花蕙蘭疫病的棕櫚疫黴菌進行生長特性的研究。結果錶明:不同培養基對該菌生長影響不同,菌絲在V8汁培養基上生長最快,在清水瓊脂培養基上生長最慢,不同培養基上菌落平均直徑依次是:V8汁>大花蕙蘭葉煎汁﹥番茄汁> PDA= PSA>玉米粉>清水瓊脂,最大與最小菌落平均直徑差為3.23 cm ,且菌落形態有差異;碳源和氮源以甘露醇和牛肉膏最佳;病原菌在15~35℃, pH3~10的條件下均能生長,最適的生長溫度為30℃,最適的p H值為6;菌絲的緻死溫度為50℃;光暗交替有利于該菌的生長。
대인기복건지구대화혜란역병적종려역매균진행생장특성적연구。결과표명:불동배양기대해균생장영향불동,균사재V8즙배양기상생장최쾌,재청수경지배양기상생장최만,불동배양기상균락평균직경의차시:V8즙>대화혜란협전즙﹥번가즙> PDA= PSA>옥미분>청수경지,최대여최소균락평균직경차위3.23 cm ,차균락형태유차이;탄원화담원이감로순화우육고최가;병원균재15~35℃, pH3~10적조건하균능생장,최괄적생장온도위30℃,최괄적p H치위6;균사적치사온도위50℃;광암교체유리우해균적생장。
Growth characteristics of Phytophthora palmivora that causes the cymbidium blight in Fujian were studied .The results showed that (a) the pathogen grew differently on different media ,(b) the bacteria grew the fastest on V8 juice ,and the slowest on water‐agar among various media , (c) the bacterial colonies formed on different media differed in size in the order of V8 juice>cymbidium leaf extract> tomato juice> PDA= PSA>corn flour> water‐agar , (d) the averaged difference between the largest and the smallest colonies was 3.23 cm in diameter ,(e) the morphology of the colonies grown on different media varied appreciably ,(f) beef extract seemed to be the best nitrogen source for the bacterial growth ,while mannitol the best for carbon ,(g) the pathogen could grow in the range of 15-35℃ and pH 3 -10 ,with the optimum temperature at 30℃and pH of 6 ,(h) the lethal temperature for the mycelia was 50℃ ,and (i) the bacteria seemed to thrive under alternated light and dark conditions .