CAJ | 학술논문

为获得高产杆菌肽菌株，对海水养殖环境中分离的产杆菌肽菌株地衣芽孢杆菌ZZ061进行A T RP诱变，以藤黄微球菌为指示菌，采用抑菌圈法进行筛选，获得一株产杆菌肽水平明显提高的诱变株S F1406，其摇瓶效价比野生菌株提高130％，达到560U·mL －1。采用正交试验优化S F1406产杆菌肽培养基，结果表明：在玉米淀粉含量为3％，硫酸铵含量为1.5％，豆饼粉含量为1％，蔗糖含量为3％时效果最佳，杆菌肽效价进一步提高44％，达到810U·mL －1。
위획득고산간균태균주，대해수양식배경중분리적산간균태균주지의아포간균ZZ061진행A T RP유변，이등황미구균위지시균，채용억균권법진행사선，획득일주산간균태수평명현제고적유변주S F1406，기요병효개비야생균주제고130％，체도560U·mL －1。채용정교시험우화S F1406산간균태배양기，결과표명：재옥미정분함량위3％，류산안함량위1.5％，두병분함량위1％，자당함량위3％시효과최가，간균태효개진일보제고44％，체도810U·mL －1。
The bacitracin‐producing Bacillus licheniformis ZZ061 was isolated from an aquaculture facility .From the isolated bacteria , mutants with enhanced capability of generating bacitrcin were bred by means of ARTP mutagenesis .In the process ,Micrococcus luteus was used as the indicator bacterium ,and the bacteriostatic circle method employed for the screening .One of the mutants ,SF1406 ,showed the greatest increase ,i .e .,130% higher than the wild strain , in its bacitrcin productivity . Subsequently , an orthogonal experiment was conducted to optimize the culture medium formulation .It was found that the medium consisting of 3% corn starch ,1.5%ammonium sulfate ,1% soybean powder and 3% sucrose rendered the most desirable results with an additional 44%productivity increase on the yield of 810 U · mL -1 .