CAJ | 학술논문

利用自行研制的旋转装置和紫外灯管辐射培养朱砂根的愈伤组织，研究模拟空间环境对愈伤组织岩白菜素合成的影响，以期寻找到有利于岩白菜素合成和积累的环境因子。研究结果表明：经旋转处理的朱砂根愈伤组织岩白菜素含量明显提高，且峰值出现的时间比对照提前，峰值是对照的1.85倍；波长为308 nm的紫外线辐射对愈伤组织培养前期的岩白菜素积累有利。本研究明确了2种模拟空间环境对朱砂根愈伤组织岩白菜素合成有不同的促进作用，但对愈伤组织的生长都有抑制作用，将为朱砂根愈伤组织岩白菜素的进一步利用奠定基础。
이용자행연제적선전장치화자외등관복사배양주사근적유상조직，연구모의공간배경대유상조직암백채소합성적영향，이기심조도유리우암백채소합성화적루적배경인자。연구결과표명：경선전처리적주사근유상조직암백채소함량명현제고，차봉치출현적시간비대조제전，봉치시대조적1.85배；파장위308 nm적자외선복사대유상조직배양전기적암백채소적루유리。본연구명학료2충모의공간배경대주사근유상조직암백채소합성유불동적촉진작용，단대유상조직적생장도유억제작용，장위주사근유상조직암백채소적진일보이용전정기출。
A lab‐designed clinostat and a UV lamp were applied to enhance the synthesis and accumulation of bergenin in the culture of A rdisia crenata Sims . calli . The simulated microgravity significantly increased the bergenin content with a peak amount 1.85 times of that of control . It also hastened the bergenin formation shortening the time to reach its peak in the calli .The exposure of calli to UV at 308 nm during the early stage of the culture benefited the bergenin accumulation .Both treatments appeared to induce varied effects in promoting the bergenin synthesis and accumulation .However ,at the same time ,they also retarded the callus growth .