CAJ | 학술논문

以葛根粉为原料,采用乙醇提取法,研究葛根中黄酮的提取及纯化工艺。采用紫外分光光度法测定黄酮标准曲线用于计算葛根中黄酮含量。在乙醇提取法提取葛根黄酮的研究中,以提取时间、料液比、乙醇体积分率、温度为考察因素,进行单因素条件研究。结果表明,最佳提取时间为1.5 h,料液比为1∶13 g/mL,乙醇体积分率为40%,温度为60℃,葛根黄酮的提取率达到2.64 mg/g。以LS-303型大孔吸附树脂为吸附剂,以葛根黄酮在该树脂上的最佳动态吸附与解吸条件为操作参数,采用树脂柱进行葛根提取液中黄酮的分离纯化,采用紫外分光光度法检验纯化效果,研究发现LS-303型树脂对葛根黄酮有良好的纯化作用。
이갈근분위원료,채용을순제취법,연구갈근중황동적제취급순화공예。채용자외분광광도법측정황동표준곡선용우계산갈근중황동함량。재을순제취법제취갈근황동적연구중,이제취시간、료액비、을순체적분솔、온도위고찰인소,진행단인소조건연구。결과표명,최가제취시간위1.5 h,료액비위1∶13 g/mL,을순체적분솔위40%,온도위60℃,갈근황동적제취솔체도2.64 mg/g。이LS-303형대공흡부수지위흡부제,이갈근황동재해수지상적최가동태흡부여해흡조건위조작삼수,채용수지주진행갈근제취액중황동적분리순화,채용자외분광광도법검험순화효과,연구발현LS-303형수지대갈근황동유량호적순화작용。
Based on the Kudzu root powder as the research object,using the ultraviolet spectrophotometric method,standard curve of flavonoids is used to calculate Kudzu root flavonoids content. In the study of ethanol extraction method to extract flavonoids of Kudzu root,extracting duration,solid-to-solvent ratio,al-cohol volume fraction and temperature were investigate factors by one-factor-at-a-time methods. Results showed that the optimum extracting time was 1. 5 h,and at a solid-to-solvent 1∶13(g/mL),alcohol vol-ume fraction was 40%,temperature was 60 ℃,the extraction yield was 2. 64 mg/g. LS-303 macroporous adsorption resin was adsorbent. The operation was in optimum condition of dynamic adsorption and de-sorption. It used resin column to separate and purify flavonoids of Kudzu root and UV spectrophotometry testing to examine purification effect. Study found that the LS-303 resin in the separation and purification of flavonoids of Kudzu root had good effect.