CAJ | 학술논문

目的：探讨甲胎蛋白（AFP）变异体 L2（AFP‐L2）在孕妇唐氏综合征筛查中的意义，为其在临床应用提供依据。方法选取250例健康孕妇及22例唐氏综合征孕妇采集血清标本，采用功能磁球分离捕获AFP‐L2，采用时间分辨荧光免疫法检测AFP及AFP‐L2水平并计算AFP‐L2百分率（AFP‐L2％）。结果唐氏综合征孕妇血清AFP水平[（20．2±4．2）ng／mL]低于健康孕妇血清AFP水平[（46．7±19．9）ng／mL]，差异有统计学意义（P＜0．05）；唐氏综合征孕妇血清AFP‐L2％高于健康孕妇，差异有统计学意义（P＜0．05）。结论检测孕妇血清AFP水平及AFP‐L2％的变化可作为唐氏综合征筛查的一项指标。
목적：탐토갑태단백（AFP）변이체 L2（AFP‐L2）재잉부당씨종합정사사중적의의，위기재림상응용제공의거。방법선취250례건강잉부급22례당씨종합정잉부채집혈청표본，채용공능자구분리포획AFP‐L2，채용시간분변형광면역법검측AFP급AFP‐L2수평병계산AFP‐L2백분솔（AFP‐L2％）。결과당씨종합정잉부혈청AFP수평[（20．2±4．2）ng／mL]저우건강잉부혈청AFP수평[（46．7±19．9）ng／mL]，차이유통계학의의（P＜0．05）；당씨종합정잉부혈청AFP‐L2％고우건강잉부，차이유통계학의의（P＜0．05）。결론검측잉부혈청AFP수평급AFP‐L2％적변화가작위당씨종합정사사적일항지표。
Objective To explore significance of alpha‐fetoprotein isoform L2(AFP‐L2) in the screening of Down syndrome in pregnant women ,so as to provide references for clinical application .Methods A total of 250 healthy pregnant women and 22 preg‐nant women with Down syndrome were enrolled in this study .Serum specimens were collected and AFP‐12 was separated and cap‐tured by using the magnetic bal ,time‐resolved fluorescence immunoassay was used to detect levels of AFP and AFP‐L2 ,and the percentage of AFP‐L2 (AFP‐L2% ) was calculated .Results The serum level of AFP of pregnant women with Down syndrome [(20.2±4.2)ng/mL]was lower than that of healthy pregnant women[(46.7±19.9)ng/mL],and had statistically significant difference(P<0 .05) .Serum AFP‐L2% of pregnant women with Down syndrome was higher than that of healthy pregnant women , and had statistically significant difference(P<0 .05) .Conclusion Detection of AFP level and AFP‐L2% could be an indicator for Dow n syndrome screening .