CAJ | 학술논문

目的：探讨直接抗人球蛋白试验（DAT）阳性患者的交叉配血方法。方法采用 DAT 法筛查红细胞表面的不规则抗体，微柱凝胶法进行血型鉴定，凝聚胺法和抗人球蛋白法交叉配血。结果共计5例 DAT 阳性的疑难配血患者，其血型分别为 A-DCcEe，O-DCCee，O-DCcee，A-DCCee 和 A-DCcee。选择相应血型抗原阴性的供者血样进行交叉配血，凝集强度均弱于自身对照，建议观察输注洗涤红细胞，患者未发生不良反应。结论对于 DAT 阳性的患者，可配合性输注相应血型抗原阴性的洗涤红细胞。
목적：탐토직접항인구단백시험（DAT）양성환자적교차배혈방법。방법채용 DAT 법사사홍세포표면적불규칙항체，미주응효법진행혈형감정，응취알법화항인구단백법교차배혈。결과공계5례 DAT 양성적의난배혈환자，기혈형분별위 A-DCcEe，O-DCCee，O-DCcee，A-DCCee 화 A-DCcee。선택상응혈형항원음성적공자혈양진행교차배혈，응집강도균약우자신대조，건의관찰수주세조홍세포，환자미발생불량반응。결론대우 DAT 양성적환자，가배합성수주상응혈형항원음성적세조홍세포。
Objective To investigate the appropriate cross-matching methods for the direct antiglobulin test (DAT)positive patients.Methods The DAT was used to screen the irregular antibodies on the surface of erythrocytes.The microcolumn gel test was used for blood identification.The antiglobulin test and manual polybrene test were used for cross-matching.Re-sults A total of five DAT positive cases were collected in study.The blood groups of each case were detected,including A-DCcEe,O-DCCee,O-DCcee,A-DCCee and A-DCcee.The donor samples with corresponding blood-antigen negative were cho-sen for cross-matching,and the agglutination intensity of each cross-matching tube was weaker than the control.The transfu-sion of washed red blood cells was suggested.Finally,each transfusion was safe and efficient.Conclusion The infusion of the washed red blood cells with corresponding blood-antigen negative could efficiently ensure the safety of blood transfusion.