草业学报
草業學報
초업학보
Acta Prataculturae Sinica
2015年
9期
96-103
,共8页
刘莎莎%程园园%张丹%王晓丹%刘佳莉%郭长虹
劉莎莎%程園園%張丹%王曉丹%劉佳莉%郭長虹
류사사%정완완%장단%왕효단%류가리%곽장홍
芽孢杆菌%紫花苜蓿%生物防治%尖孢镰刀菌%根腐病
芽孢桿菌%紫花苜蓿%生物防治%尖孢鐮刀菌%根腐病
아포간균%자화목숙%생물방치%첨포렴도균%근부병
Bacillus spp.%alfalfa (Medicago sativa)%biocontrol%Fusarium oxysporum%root rot
从紫花苜蓿根际土中分离出2株芽孢杆菌(Bacillus spp.)菌株 CYY-6和 CYY-42,并评价了其对尖孢镰刀菌(Fusarium oxysporum )的抑制作用,及其对由尖孢镰刀菌引起的苜蓿根腐病的防治效果。经过形态观察、16S rD-NA 序列分析和生理生化鉴定,确定菌株 CYY-6为地衣芽孢杆菌(Bacillus licheniformis ),CYY-42为短短芽孢杆菌(Brevibacillus brevis )。同时检测发现这2株芽孢杆菌均能不同程度地合成嗜铁素。在平板对峙实验中,2株菌株均能对尖孢镰刀菌产生拮抗作用,并产生抑菌带,抑菌率分别为49.3%和56.3%。且其发酵液可以致使尖孢镰刀菌菌丝及孢子生长异常,出现菌丝扭曲、断裂、破碎,孢子数量明显减少等现象。在盆栽实验中,2株菌株对由尖孢镰刀菌引起的紫花苜蓿根腐病的相对防效分别为57.55%和64.03%,在紫花苜蓿根腐病防治方面具有较好的应用潜力。
從紫花苜蓿根際土中分離齣2株芽孢桿菌(Bacillus spp.)菌株 CYY-6和 CYY-42,併評價瞭其對尖孢鐮刀菌(Fusarium oxysporum )的抑製作用,及其對由尖孢鐮刀菌引起的苜蓿根腐病的防治效果。經過形態觀察、16S rD-NA 序列分析和生理生化鑒定,確定菌株 CYY-6為地衣芽孢桿菌(Bacillus licheniformis ),CYY-42為短短芽孢桿菌(Brevibacillus brevis )。同時檢測髮現這2株芽孢桿菌均能不同程度地閤成嗜鐵素。在平闆對峙實驗中,2株菌株均能對尖孢鐮刀菌產生拮抗作用,併產生抑菌帶,抑菌率分彆為49.3%和56.3%。且其髮酵液可以緻使尖孢鐮刀菌菌絲及孢子生長異常,齣現菌絲扭麯、斷裂、破碎,孢子數量明顯減少等現象。在盆栽實驗中,2株菌株對由尖孢鐮刀菌引起的紫花苜蓿根腐病的相對防效分彆為57.55%和64.03%,在紫花苜蓿根腐病防治方麵具有較好的應用潛力。
종자화목숙근제토중분리출2주아포간균(Bacillus spp.)균주 CYY-6화 CYY-42,병평개료기대첨포렴도균(Fusarium oxysporum )적억제작용,급기대유첨포렴도균인기적목숙근부병적방치효과。경과형태관찰、16S rD-NA 서렬분석화생리생화감정,학정균주 CYY-6위지의아포간균(Bacillus licheniformis ),CYY-42위단단아포간균(Brevibacillus brevis )。동시검측발현저2주아포간균균능불동정도지합성기철소。재평판대치실험중,2주균주균능대첨포렴도균산생길항작용,병산생억균대,억균솔분별위49.3%화56.3%。차기발효액가이치사첨포렴도균균사급포자생장이상,출현균사뉴곡、단렬、파쇄,포자수량명현감소등현상。재분재실험중,2주균주대유첨포렴도균인기적자화목숙근부병적상대방효분별위57.55%화64.03%,재자화목숙근부병방치방면구유교호적응용잠력。
The aims of this study were to identify two Bacillus spp.,CYY-6 and CYY-42,isolated from the rhizosphere of alfalfa,and to evaluate their ability to inhibit Fusarium oxysporum,the causal pathogen of alfal-fa root rot.Based on physiological,biochemical,morphological,and 16S rDNA sequencing analyses,strain CYY-6 was identified as Bacillus licheniformis and CYY-42 as Brevibacillus brevis.Both strains were able to synthesize siderophores,although at different levels.In a dual culture assay,both strains inhibited the growth of F.oxysporum.Based on the inhibition zones,the inhibitory rates of CYY-6 and CYY-42 against F.oxyspo-rum were 49.3% and 56.3%,respectively.The fermentation broth of each strain resulted in deformed growth of the mycelia and spores of F.oxysporum.The mycelia were twisted,broken,or fragmented,and the num-ber of spores was significantly decreased.Strains CYY-6 and CYY-42 showed 57.55% and 64.03% inhibition of alfalfa root rot in pot experiments,indicating that both strains have potential applications in controlling alfal-fa root rot.