CAJ | 학술논문

内生真菌与植物所形成的共生体普遍存在于冷季禾草中，共生体有利于提高宿主植物的生物和非生物抗性。虽然目前研究者们已对禾草内生真菌做了大量的研究，但是尚未发现对分布于中国西北地区披碱草属植物无性世代 Neotyphodium 内生真菌培养特征的研究。本研究从中国西北地区分离出15株来自不同地理种群披碱草属植物无性世代 Neotyphodium 内生真菌。通过分离培养，测定了这15株无性世代 Neotyphodium 内生真菌的菌落生长速度，结果显示，这15株无性世代 Neotyphodium 内生真菌菌落的平均生长速度为（0．41～0．85）mm／d，且生长速度与海拔具有相关性。分布在海拔低于3000 m 的无性世代 Neotyphodium 内生真菌的菌落生长速度比生长于海拔高于3000 m 菌落的生长速度快。观察菌落发现，分布在海拔高于3000 m 的菌落正面特征较为一致，而分布在海拔低于3000 m 的菌落正面特征出现不同。另外，通过多基因联合建树发现，分布在海拔高于3000 m 的无性世代 Neotyphodium 内生真菌聚在一个分支，而分布在海拔低于3000 m 的无性世代 Neotyphodium 内生真菌呈现星状分布。由于目前尚未对中国西北地区披碱草属植物无性世代 Neotyphodium 内生真菌命名。因此，希望本研究结果能为该菌的命名提供理论依据。
내생진균여식물소형성적공생체보편존재우랭계화초중，공생체유리우제고숙주식물적생물화비생물항성。수연목전연구자문이대화초내생진균주료대량적연구，단시상미발현대분포우중국서북지구피감초속식물무성세대 Neotyphodium 내생진균배양특정적연구。본연구종중국서북지구분리출15주래자불동지리충군피감초속식물무성세대 Neotyphodium 내생진균。통과분리배양，측정료저15주무성세대 Neotyphodium 내생진균적균락생장속도，결과현시，저15주무성세대 Neotyphodium 내생진균균락적평균생장속도위（0．41～0．85）mm／d，차생장속도여해발구유상관성。분포재해발저우3000 m 적무성세대 Neotyphodium 내생진균적균락생장속도비생장우해발고우3000 m 균락적생장속도쾌。관찰균락발현，분포재해발고우3000 m 적균락정면특정교위일치，이분포재해발저우3000 m 적균락정면특정출현불동。령외，통과다기인연합건수발현，분포재해발고우3000 m 적무성세대 Neotyphodium 내생진균취재일개분지，이분포재해발저우3000 m 적무성세대 Neotyphodium 내생진균정현성상분포。유우목전상미대중국서북지구피감초속식물무성세대 Neotyphodium 내생진균명명。인차，희망본연구결과능위해균적명명제공이론의거。
Forage grasses (Poaceae)often harbour endophytes belonging to the Neotyphodium and Epichlo(Clavicipitaceae,Ascomycota)genera.Neotyphodium endophytes,the asexual state of Epichlospecies,helps to protect cool-season grasses against biotic and abiotic stresses including drought,disease,herbivory and para-sitism and enhance aboveground and belowground vegetative and reproductive growth.There is little informa-tion on colonial diversity of asexual endophytes isolated from Elymus species in northwest Chinese.In the present study,different geographic populations of Elymus species were tested using the aniline blue screening method;15 endophyte strains were isolated from endophyte positive plants.The average growth rate (on pota-to dextrose agar)of the isolated strains was 0.41-0.85 mm/day and was correlated with altitude.Endophyte strains isolated from regions below an altitude of 3000 m grew faster than strains isolated from regions above 3000 m.The colonial morphology of endophyte strains isolated from regions above 3000 m was similar whereas the colonial morphology of endophyte strains isolated from lower altitudes differed.In addition,the phyloge-netic trees showed that endophytes collected above 3000 m were clustered whereas endophyte strains from low-er altitudes demonstrated a star-like topology.The results will provide a theoretical basis for the identification of endophyte from northwest Chinese Elymus species.