草业学报
草業學報
초업학보
Acta Prataculturae Sinica
2015年
9期
73-79
,共7页
γ-zein 基因%菊苣%农杆菌转化%含硫氨基酸
γ-zein 基因%菊苣%農桿菌轉化%含硫氨基痠
γ-zein 기인%국거%농간균전화%함류안기산
γ-zein genes%chicory (Cichorium intybus )%agrobacterium-mediated transformation%sulphur-amino acid
含硫氨基酸具有动物营养与免疫相关的重要生理功能，为提高菊苣中含硫氨基酸含量，采用根癌农杆菌介导法将玉米种子贮藏蛋白含硫氨基酸基因γ-zein 和绿色荧光蛋白 GFP 融合基因转入到菊苣无菌苗叶片中，经过共培养、潮霉素抗性筛选、分化、再生和炼苗，得到抗性植株。对抗性植株进行 PCR、PCR-Southern、斑点杂交和 RT－PCR 分析，结果表明，外源目的基因已经整合到菊苣基因组中并且得到了表达，为提高菊苣含硫氨基酸含量，改善其品质奠定了基础。
含硫氨基痠具有動物營養與免疫相關的重要生理功能，為提高菊苣中含硫氨基痠含量，採用根癌農桿菌介導法將玉米種子貯藏蛋白含硫氨基痠基因γ-zein 和綠色熒光蛋白 GFP 融閤基因轉入到菊苣無菌苗葉片中，經過共培養、潮黴素抗性篩選、分化、再生和煉苗，得到抗性植株。對抗性植株進行 PCR、PCR-Southern、斑點雜交和 RT－PCR 分析，結果錶明，外源目的基因已經整閤到菊苣基因組中併且得到瞭錶達，為提高菊苣含硫氨基痠含量，改善其品質奠定瞭基礎。
함류안기산구유동물영양여면역상관적중요생리공능，위제고국거중함류안기산함량，채용근암농간균개도법장옥미충자저장단백함류안기산기인γ-zein 화록색형광단백 GFP 융합기인전입도국거무균묘협편중，경과공배양、조매소항성사선、분화、재생화련묘，득도항성식주。대항성식주진행 PCR、PCR-Southern、반점잡교화 RT－PCR 분석，결과표명，외원목적기인이경정합도국거기인조중병차득도료표체，위제고국거함류안기산함량，개선기품질전정료기출。
Sulfur-containing amino acids have important physiological functions related to animal nutrition and immunity.To improve the sulfur-amino acid content of chicory,leaves of chicory were transformed with the Sulphur-amino acid gene γ-zein,an important prolamin storage protein from Zea mays and a green fluorescent protein (GFP)gene using Agrobacterium mediated transfusion.After co-culture,selective differentiation and regeneration,hygromycin resistant plants were obtained.Resistant plants were detected using PCR,PCR-southern,dot blot hybridization and RT-PCR.The results demonstrated that the γ-zein genes had been inte-grated into the genome of chicory and expressed on a nucleic acid level in the transgenic plants.
