CAJ | 학술논문

目的：对比间接免疫荧光（IFA）与实时荧光聚合酶链反应（PCR）检测小儿支原体肺炎的效果。方法选取137例临床诊断的肺炎支原体（M P ）感染患儿，按年龄划分为＜1岁（35例）、1～＜5岁（69例）、5～15岁（33例），采集血液标本和咽拭子，分别采用IFA和实时荧光PCR进行检测。同时对纳入的所有患儿行支原体肺炎的常规治疗，以治疗有效为判断M P感染的标准，按年龄段统计分析两种方式检测的阳性符合率。结果＜1岁和1～＜5岁患儿实时荧光PCR检测阳性符合率高于IFA ，5～15岁患儿IFA检测阳性率高于实时荧光PCR ，差异均有统计学意义（P＜0．05）；而两种方式检测总阳性符合率比较差异无统计学意义（P＞0．05）。结论 IFA与实时荧光PCR都可作为检测MP的有效方式，但检测效果在不同年龄段存在一定的差异，建议对5岁以下患儿采用实时荧光PCR进行检测，5岁及以上患儿则可选择IFA检测，以提高检测的准确度，更好地为临床用药提供指导。
목적：대비간접면역형광（IFA）여실시형광취합매련반응（PCR）검측소인지원체폐염적효과。방법선취137례림상진단적폐염지원체（M P ）감염환인，안년령화분위＜1세（35례）、1～＜5세（69례）、5～15세（33례），채집혈액표본화인식자，분별채용IFA화실시형광PCR진행검측。동시대납입적소유환인행지원체폐염적상규치료，이치료유효위판단M P감염적표준，안년령단통계분석량충방식검측적양성부합솔。결과＜1세화1～＜5세환인실시형광PCR검측양성부합솔고우IFA ，5～15세환인IFA검측양성솔고우실시형광PCR ，차이균유통계학의의（P＜0．05）；이량충방식검측총양성부합솔비교차이무통계학의의（P＞0．05）。결론 IFA여실시형광PCR도가작위검측MP적유효방식，단검측효과재불동년령단존재일정적차이，건의대5세이하환인채용실시형광PCR진행검측，5세급이상환인칙가선택IFA검측，이제고검측적준학도，경호지위림상용약제공지도。
Objective To compare efficacy of indirect immunofluorescence (IFA) and real‐time fluorescent polymerase chain re‐action(PCR) in detection of mycoplasma pneumonia in children .Methods A total of 137 children clinically diagnosed as Mycoplas‐ma pneumoniae(MP) infection were selected and divided into groups by age ,including <1 years old group(35 cases) ,1- <5 years old group(69 cases) and 5-15 years old group(33 cases) .Blood specimen and throat swabs were collected and detected by using IFA and real‐time fluorescent PCR .At the same time ,all of the selected children were treated with conventional therapy ,according to total effective rate ,positive coincidence rates of the two methods were statistically analysed by age .Results The positive coinci‐dence rates in children with MP infection <1 years old and 1- <5 years old detected by using real‐time fluorescent PCR were high‐er than that detected by using IFA ,while among children 5-15 years old ,the positive coincidence rate was higher detected by using IFA compared with that detected by using real‐time fluorescent PCR ,all had statistically significant differences (P<0 .05) .The o‐verall positive coincidence rates of the two methods were not significantly different(P>0 .05) .Conclusion IFA and real‐time fluo‐rescent PCR both could be used as effective methods for detecting MP ,but there are some differences of detective efficacy between the two methods in each age group .Therefore ,it is suggested that for children under 5 years old real‐time fluorescent PCR might be selected ,for children aged 5 years old and over IFA might be selected ,in order to improve the detection accuracy and provide better guidance to clinical medication .