CAJ | 학술논문

目的：评估 Beckman Coulter 实验室自动化系统生化免疫样品间携带污染情况，探讨不选择分杯模块时生化免疫共用一管血的可行性。方法将高浓度及不同样品量（2000μl，500μl）低浓度的 HCG 样品按照一定顺序在 Beckman AU5421全自动生化分析仪上检测生化项目，比较检测生化项目前后低浓度样品 HCG 含量的变化评估生化免疫样品间携带污染情况，并在增加 Beckman AU5421全自动生化分析仪特殊清洗程序后重复试验。结果在不同样品量（2000μl，500μl）条件下，Beckman AU5421全自动生化分析仪单模块及整机携带污染分别为5.44，15.47，23.51，45.96 ppm，不同样品量及单模块与整机间携带污染差异均有统计学意义（t ＝14.553，P ＜0.001；t ＝5.527，P ＝0.005；t ＝3.985，P ＝0.016；t＝20.457，P ＜0.001）；增加 Beckman AU5421特殊清洗程序后仅检出500μl 样品量时整机携带污染为0.22 ppm。结论不选择分杯模块时，Beckman Coulter 实验室自动化系统可使用特殊清洗程序解决生化免疫间携带污染。
목적：평고 Beckman Coulter 실험실자동화계통생화면역양품간휴대오염정황，탐토불선택분배모괴시생화면역공용일관혈적가행성。방법장고농도급불동양품량（2000μl，500μl）저농도적 HCG 양품안조일정순서재 Beckman AU5421전자동생화분석의상검측생화항목，비교검측생화항목전후저농도양품 HCG 함량적변화평고생화면역양품간휴대오염정황，병재증가 Beckman AU5421전자동생화분석의특수청세정서후중복시험。결과재불동양품량（2000μl，500μl）조건하，Beckman AU5421전자동생화분석의단모괴급정궤휴대오염분별위5.44，15.47，23.51，45.96 ppm，불동양품량급단모괴여정궤간휴대오염차이균유통계학의의（t ＝14.553，P ＜0.001；t ＝5.527，P ＝0.005；t ＝3.985，P ＝0.016；t＝20.457，P ＜0.001）；증가 Beckman AU5421특수청세정서후부검출500μl 양품량시정궤휴대오염위0.22 ppm。결론불선택분배모괴시，Beckman Coulter 실험실자동화계통가사용특수청세정서해결생화면역간휴대오염。
Objective To evaluate the Carryover between the chemistry and immunoassay in Beckman Coulter Laboratory Au-tomation System and decide to whether sharing samples for testing between chemistry and immunoassay systems or not. Methods According to a certain order,high concentration samples and low concentration samples of HCG with different sample volume (500 μl,2 000 μl)were tested on Beckman AU5421 automatic biochemical analyzer.The HCG of low concen-tration samples were then tested to evaluate the carryover between the chemistry and immunoassay and explored the correc-tive procedure to deal with the carryover by increasing special cleaning process of beckman AU5421 automatic biochemistry analyzer.Results Under different sample volume,the carryover in a single module and as a whole of the beckman AU5421 automatic biochemistry analyzer were 5.44,15.47,23.51 and 45.96 ppm respectively (t=14.553,P <0.001;t=5.527,P =0.005;t=3.985,P =0.016;t=20.457,P <0.001).By increasing special cleaning process the carryover of 0.22 ppm was detected in 500 μl sample volume of the beckman AU5421 automatic biochemistry analyzer as a whole.Conclusion The car-ryover between the chemistry and immunoassay in Beckman Coulter Laboratory Automation System could been sovled by in-creasing special cleaning process of beckman AU5421 automatic biochemistry analyzer.