CAJ | 학술논문

目的：探讨去白细胞悬浮红细胞储血袋两种不同放置方式对上清液游离血红蛋白（FHb）和红细胞溶血率的影响，保证临床输血的安全、有效。方法选择20例无偿献血者每人400 ml 的全血，24 ml 内制成去白细胞悬浮红细胞制品，将其等量均匀分装成10袋，随机分成2组，每组5袋，一组以直立放置，一组以水平放置，在同一环境条件下储存，分别在储存的第7天，14，21，28，35天时随机从各组取出1袋血液，分别测定其 FHb 和红细胞溶血率，进行统计学分析。结果FHb 和红细胞溶血率在贮存的第21天时检测的结果，直立放置组分别为（217.310±48.477）mg／L 和（0.250±0.056）％，水平放置组分别为（173.972±39.027）mg／L 和（0.189±0.045）％，直立放置组的结果要高于水平放置组，统计学结果分别为 t＝3.114，P ＝0.003＜0.05和 t＝3.798，P ＝0.001＜0.05，差异有统计学意义。结论在血液储存期内，储血袋水平方式放置可减少红细胞破坏，对血液的贮存更加有利。
목적：탐토거백세포현부홍세포저혈대량충불동방치방식대상청액유리혈홍단백（FHb）화홍세포용혈솔적영향，보증림상수혈적안전、유효。방법선택20례무상헌혈자매인400 ml 적전혈，24 ml 내제성거백세포현부홍세포제품，장기등량균균분장성10대，수궤분성2조，매조5대，일조이직립방치，일조이수평방치，재동일배경조건하저존，분별재저존적제7천，14，21，28，35천시수궤종각조취출1대혈액，분별측정기 FHb 화홍세포용혈솔，진행통계학분석。결과FHb 화홍세포용혈솔재저존적제21천시검측적결과，직립방치조분별위（217.310±48.477）mg／L 화（0.250±0.056）％，수평방치조분별위（173.972±39.027）mg／L 화（0.189±0.045）％，직립방치조적결과요고우수평방치조，통계학결과분별위 t＝3.114，P ＝0.003＜0.05화 t＝3.798，P ＝0.001＜0.05，차이유통계학의의。결론재혈액저존기내，저혈대수평방식방치가감소홍세포파배，대혈액적저존경가유리。
Objective Discussion leukoreduction of red blood cells suspended in two different storage bag placement and he-molysis rate impact on the supernatant free hemoglobin (FHb),to ensure the clinical transfusion is safe and effective.Meth-ods Selected 20 donors to sample 400 ml whole blood per person to make leukodepleted red blood cells,which were evenly divided into 10 bags.The 10 bags were randomly divided into two groups,one to the upright position,one group of horizon-tal.The two groups were stored under the same conditions.Respectively,in the 7,14,21,28,35 day,randomly removed one storage bag from each group,FHb and red blood cell hemolysis rate were measured and analyzed statistically.Results FHb and hemolysis rate results stored in the first 21 days of testing,uprightgroup were (217.310±48.477)mg/L and (0.250± 0.056)%,respectively horizontal group (173.972±39.027)mg/L and (0.189±0.045)%,the results set upright than hori-zontal group,the results were statistically(t=3.114,P =0.003<0.05 and t=3.798,P =0.001<0.05),the difference was statistically significant.Conclusion In the blood storage period,storage bags can be placed horizontally to reduce the de-struction of red blood cells,blood storage is more favorable.