CAJ | 학술논문

目的：探讨长期单独使用肾上腺素β2受体(β2 AR)激动剂及其与糖皮质激素联用时对气道平滑肌细胞胆碱能毒蕈碱受体3型(M3 R)的影响。方法使用免疫印迹法检测福莫特罗及糖皮质激素对原代培养的气道平滑肌细胞 M3 R 蛋白的影响。结果福莫特罗呈时间和剂量依赖性上调 M3 R 蛋白的表达，布地奈德能够抑制福莫特罗上调 M3 R 的作用。结论长期规律使用肾上腺素β2受体(β2 AR)激动剂导致严重哮喘恶化的风险增加可能与其上调 M3 R 蛋白有关，而糖皮质激素与其联用能够抑制这一作用。
목적：탐토장기단독사용신상선소β2수체(β2 AR)격동제급기여당피질격소련용시대기도평활기세포담감능독심감수체3형(M3 R)적영향。방법사용면역인적법검측복막특라급당피질격소대원대배양적기도평활기세포 M3 R 단백적영향。결과복막특라정시간화제량의뢰성상조 M3 R 단백적표체，포지내덕능구억제복막특라상조 M3 R 적작용。결론장기규률사용신상선소β2수체(β2 AR)격동제도치엄중효천악화적풍험증가가능여기상조 M3 R 단백유관，이당피질격소여기련용능구억제저일작용。
Objective To investigate the potential effect of formoterol, a long-acting beta2-adrenergic agonist, and combined glucocorticoids on the expression of muscarinic M3 receptors ( M3 R) in rat airway smooth muscle cells (ASMCs). Methods Primary rat ASMCs were cultured. Protein expressions of M3 R were determined by Western blot a-nalysis after treatments of formoterol and combined glucocorticoids. Results Formoterol increased the protein expression of M3 R in rat ASMCs in a time-and dose-dependent manner. Glucocorticoid budesonide significantly suppressed formoterol-in-duced up-regulation of M3 R. Conclusions Formoterol can up-regulate M3 R protein in rat ASMCs which lead to the in-creased risks of severe asthma excerbation. Glucocorticoids can suppress M3 R protein expression.