CAJ | 학술논문

目的：比较 Vitek2 Compact 和 Walkaway 40全自动微生物鉴定系统在布鲁菌鉴定中的应用。方法临床分离菌株，采用 Vitek2 Compact 和 Walkaway 40全自动微生物鉴定系统进行鉴定，并将鉴定结果和16S rRNA 基因序列检测的结果进行比较。结果临床分离菌株经 Vitek2 Compact GN 鉴定卡鉴定为马耳他布鲁菌，Walkaway 40 NC31鉴定卡鉴定为动物溃疡威克斯菌、莫拉菌属等。16S rRNA 基因序列分析表明，该临床分离菌株的16S rRNA 基因序列与布鲁菌匹配，排除动物溃疡威克斯菌、莫拉菌属等的可能，确定该菌株为马耳他布鲁菌。结论Vitek2 Compact 可以准确的鉴定出布鲁菌，经 Vitek2 Compact 鉴定为布鲁菌后，可采用分子生物学方法进行佐证，大大地提高了布鲁菌的检出率，降低误诊的可能性。Walkaway 40无法准确的鉴定出布鲁菌，误鉴定会导致错误的治疗并使工作人员处于实验室获得性感染的危险之中。建议使用 Walkaway 40的实验室在鉴定出动物溃疡威克斯菌或莫拉菌属等细菌时应谨慎报告，并使用 Vitek2 Com-pact 或分子生物学方法进行复查。
목적：비교 Vitek2 Compact 화 Walkaway 40전자동미생물감정계통재포로균감정중적응용。방법림상분리균주，채용 Vitek2 Compact 화 Walkaway 40전자동미생물감정계통진행감정，병장감정결과화16S rRNA 기인서렬검측적결과진행비교。결과림상분리균주경 Vitek2 Compact GN 감정잡감정위마이타포로균，Walkaway 40 NC31감정잡감정위동물궤양위극사균、막랍균속등。16S rRNA 기인서렬분석표명，해림상분리균주적16S rRNA 기인서렬여포로균필배，배제동물궤양위극사균、막랍균속등적가능，학정해균주위마이타포로균。결론Vitek2 Compact 가이준학적감정출포로균，경 Vitek2 Compact 감정위포로균후，가채용분자생물학방법진행좌증，대대지제고료포로균적검출솔，강저오진적가능성。Walkaway 40무법준학적감정출포로균，오감정회도치착오적치료병사공작인원처우실험실획득성감염적위험지중。건의사용 Walkaway 40적실험실재감정출동물궤양위극사균혹막랍균속등세균시응근신보고，병사용 Vitek2 Com-pact 혹분자생물학방법진행복사。
Objective To compare Vitek2 Compact and Walkaway 40 in the identification of Brucella.Methods Used Vitek2 Compact and Walkaway 40 automated microbial identification system to identify clinical isolates and compared with the de-tection of 16S rRNA gene sequences analysis.Results The clinical isolates was identified as Bergeyella zoohelcum or Moraxella by Walkaway 40 and as Brucella melitensis by Vitek2 Compact.16S RNA sequence analysis of the isolate,the se-quence was identical to the sequences of 16S rRNA of Brucella,which excluded the possibility of B.zoohelam and Moraxel-la .Determined that the isolate was B.melitensis.Conclusion Vitek 2 Compact can accurately identified Brucella.Use molec-ular methods to corroborate when the isolates was identified as Brucella by Vitek 2 Compact,this method can greatly im-prove the detection rate of brucellosis and reduce the possibility of misdiagnosis.Walkaway 40 cannot accurately identified Brucella,Misidentification of Brucella can result in wrong treatment of the patient and let the staff in the risk of laboratory-acquired infection.Recommend laboratory should be cautious reporting in the identified B.zoohelam or Moraxella by Walk-away 40 and use Vitek2 Compact or molecular methods for review.