现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
Journal of Modern Laboratory Medicine
2015年
4期
102-104
,共3页
肺炎支原体%白细胞介素IL-3%克拉霉素
肺炎支原體%白細胞介素IL-3%剋拉黴素
폐염지원체%백세포개소IL-3%극랍매소
mycoplasma pneumonia (MP)%interluekin-3%clarithromycin
目的：研究 IL-3在 BALB／c 小鼠肺炎支原体（MP）感染模型肺泡灌洗液和血清中的表达，阐明其与 MP 感染的关系。方法36只 BALB／c 裸鼠，随机分为3组，分别为对照组、MP 感染组和克拉霉素组，每组12只。用 PPLO 肉汤培养基 MP，将成功培养的 MP 鼻腔滴入裸鼠建立 MP 感染模型。治疗组用克拉霉素连续治疗8天，收集并分离血清，取其肺组织，收集肺泡灌洗液，采用 ELISA 法测定小鼠血清和肺泡灌洗液中 IL-3水平，肺部组织用中性甲醛固定 HE 染色，观察MP 感染小鼠肺的病变程度。结果成功建立 MP 感染小鼠模型，病理组织学观察结果显示，MP 感染 BALB／c 小鼠后炎症明显，克拉霉素治疗 BALB／c 小鼠炎症逐渐减轻。模型组血清和肺泡灌洗液中 IL-3分别为96.4±8.3 ng／L 和60.9±6.5 ng／L，与对照组比较（203.1±14.5 ng／L，96.3±12.6 ng／L），MP 感染组小鼠血清和肺泡灌洗液中 IL-3水平明显降低（t 血清＝19.50，t 肺泡＝17.20，P 值均＜0.01），用克拉霉素治疗后，小鼠血清和肺泡灌洗液中 IL-3明显升高，分别为175.6±12.5 ng／L 和85.6±10.2 ng／L，差异有统计学意义，t 血清＝17.41，t 肺泡＝14.62，P 值均＜0.01）。结论肺炎支原体感染使小鼠 IL-3水平降低，克拉霉素治疗有一定疗效，IL-3表达可能与 MP 肺炎严重程度有关。
目的：研究 IL-3在 BALB／c 小鼠肺炎支原體（MP）感染模型肺泡灌洗液和血清中的錶達，闡明其與 MP 感染的關繫。方法36隻 BALB／c 裸鼠，隨機分為3組，分彆為對照組、MP 感染組和剋拉黴素組，每組12隻。用 PPLO 肉湯培養基 MP，將成功培養的 MP 鼻腔滴入裸鼠建立 MP 感染模型。治療組用剋拉黴素連續治療8天，收集併分離血清，取其肺組織，收集肺泡灌洗液，採用 ELISA 法測定小鼠血清和肺泡灌洗液中 IL-3水平，肺部組織用中性甲醛固定 HE 染色，觀察MP 感染小鼠肺的病變程度。結果成功建立 MP 感染小鼠模型，病理組織學觀察結果顯示，MP 感染 BALB／c 小鼠後炎癥明顯，剋拉黴素治療 BALB／c 小鼠炎癥逐漸減輕。模型組血清和肺泡灌洗液中 IL-3分彆為96.4±8.3 ng／L 和60.9±6.5 ng／L，與對照組比較（203.1±14.5 ng／L，96.3±12.6 ng／L），MP 感染組小鼠血清和肺泡灌洗液中 IL-3水平明顯降低（t 血清＝19.50，t 肺泡＝17.20，P 值均＜0.01），用剋拉黴素治療後，小鼠血清和肺泡灌洗液中 IL-3明顯升高，分彆為175.6±12.5 ng／L 和85.6±10.2 ng／L，差異有統計學意義，t 血清＝17.41，t 肺泡＝14.62，P 值均＜0.01）。結論肺炎支原體感染使小鼠 IL-3水平降低，剋拉黴素治療有一定療效，IL-3錶達可能與 MP 肺炎嚴重程度有關。
목적：연구 IL-3재 BALB／c 소서폐염지원체（MP）감염모형폐포관세액화혈청중적표체，천명기여 MP 감염적관계。방법36지 BALB／c 라서，수궤분위3조，분별위대조조、MP 감염조화극랍매소조，매조12지。용 PPLO 육탕배양기 MP，장성공배양적 MP 비강적입라서건립 MP 감염모형。치료조용극랍매소련속치료8천，수집병분리혈청，취기폐조직，수집폐포관세액，채용 ELISA 법측정소서혈청화폐포관세액중 IL-3수평，폐부조직용중성갑철고정 HE 염색，관찰MP 감염소서폐적병변정도。결과성공건립 MP 감염소서모형，병리조직학관찰결과현시，MP 감염 BALB／c 소서후염증명현，극랍매소치료 BALB／c 소서염증축점감경。모형조혈청화폐포관세액중 IL-3분별위96.4±8.3 ng／L 화60.9±6.5 ng／L，여대조조비교（203.1±14.5 ng／L，96.3±12.6 ng／L），MP 감염조소서혈청화폐포관세액중 IL-3수평명현강저（t 혈청＝19.50，t 폐포＝17.20，P 치균＜0.01），용극랍매소치료후，소서혈청화폐포관세액중 IL-3명현승고，분별위175.6±12.5 ng／L 화85.6±10.2 ng／L，차이유통계학의의，t 혈청＝17.41，t 폐포＝14.62，P 치균＜0.01）。결론폐염지원체감염사소서 IL-3수평강저，극랍매소치료유일정료효，IL-3표체가능여 MP 폐염엄중정도유관。
Objective To investigate the expressions of interluekin-3 in BALB/c mice infected by mycoplasma pneumonia (MP)and to clarify the relationship between IL-3 and MP infection.Methods Total 36 BALB/c mice were randomly divid-ed into control group,MP infection group and clarithromycin treatment group.MP infection models were established with the method of dripping cultured MP into nasal cavity of mice.ELISA method was used to assay the levels of IL-3 in the the serum of the mice and bronchoalveolar lavage fluid in various groups.The lung tissue was fixed in formalin for sections,HE staining was performed for the observation of histological changes.Results The MP-infected mouse models were established successfully and MP strains were dripped into the respiratory system of mice.As showed in histopathological observasion re-sults,significantly increased after MP infection and then gradually decreased after clarithromycin treatment.The ELISA re-sults showed that the level of serum and bronchoalveolar lavage fluid of IL-3 of the mice in MP infection group were 96.4± 8.3 ng/L and 60.9 ±6.5 ng/L.Compared with the control group(203.1±14.5 ng/L,96.3±12.6ng/L),serum and bron-choalveolar lavage fluid of mice infected with MP in IL-3 levels were significantly lower (t serum = 19.50,t balf = 17.20,P <0.01),and after treatment with clarithromycin,serum and bronchoalveolar lavage fluid of IL-3 significantly increased,re-spectively(175.6±12.5 ng/L and 85.6± 10.2 ng/L,tserum = 17.41,tbalf = 14.62,P <0.01),the difference was statistically significant.Conclusion IL-3 levels were decreased in mycoplasma pneumonia mice.Clarithromycin treatment had effect on mycoplasma pneumonia infecttion.The expression level IL-3 may be related to the severity degree of MP infection.
