现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
Journal of Modern Laboratory Medicine
2015年
4期
99-101
,共3页
安哲%李思鹏%董炜%张妮%李妙羡%王香玲
安哲%李思鵬%董煒%張妮%李妙羨%王香玲
안철%리사붕%동위%장니%리묘이%왕향령
抗HBe%抗HBc%酶联免疫吸附法%化学发光微粒子免疫测定
抗HBe%抗HBc%酶聯免疫吸附法%化學髮光微粒子免疫測定
항HBe%항HBc%매련면역흡부법%화학발광미입자면역측정
anti-HBe%anti-HBc%enzyme-linked immunosorbent assay(ELISA)%chemiluminecence microparticle immunoassay (CMIA)
目的：对 ELISA 法检测 HBV-M 呈抗 HBc 阴性、抗 HBe 阳性结果的科学评价。方法ELISA 法初检 HBV-M 呈抗 HBc 阴性，抗 HBe 阳性样本105例，以 CMIA 法为参考方法复检确证抗 HBe 和抗 HBc 并评价其真阳性率和假阴性率；以其 S／CO 水平不同分组评价不同水平抗 HBe 和抗 HBc 检测结果的真实性。结果CMIA 法复检后产生3种不同结果，即抗 HBc 阳性／抗 HBe 阳性、抗 HBc 阴性／抗 HBe 阴性、抗 HBc 阳性／抗 HBe 阴性，分别占72.38％，21.91％和5.71％，抗 HBc 假阴性率和抗 HBe 真阳性率分别为78.10％和72.38％；在0.00～0.10，0.10～0.80和0.80～1.00不同 S／CO 水平内抗 HBe 真阳性率分别为42.86％，88.14％和56.25％，在1.00～1.20，1.20～2.00和2.00～3.00不同 S／CO 水平内抗 HBc 假阴性率分别为93.33％，96.15％和47.37％。结论ELISA 法检测 HBV-M 呈抗-HBc 阴性／抗 HBe 阳性结果对HBV 既往感染有重要的提示价值，但不能反映真实结果，存在三种可能，应进一步复检确证。
目的：對 ELISA 法檢測 HBV-M 呈抗 HBc 陰性、抗 HBe 暘性結果的科學評價。方法ELISA 法初檢 HBV-M 呈抗 HBc 陰性，抗 HBe 暘性樣本105例，以 CMIA 法為參攷方法複檢確證抗 HBe 和抗 HBc 併評價其真暘性率和假陰性率；以其 S／CO 水平不同分組評價不同水平抗 HBe 和抗 HBc 檢測結果的真實性。結果CMIA 法複檢後產生3種不同結果，即抗 HBc 暘性／抗 HBe 暘性、抗 HBc 陰性／抗 HBe 陰性、抗 HBc 暘性／抗 HBe 陰性，分彆佔72.38％，21.91％和5.71％，抗 HBc 假陰性率和抗 HBe 真暘性率分彆為78.10％和72.38％；在0.00～0.10，0.10～0.80和0.80～1.00不同 S／CO 水平內抗 HBe 真暘性率分彆為42.86％，88.14％和56.25％，在1.00～1.20，1.20～2.00和2.00～3.00不同 S／CO 水平內抗 HBc 假陰性率分彆為93.33％，96.15％和47.37％。結論ELISA 法檢測 HBV-M 呈抗-HBc 陰性／抗 HBe 暘性結果對HBV 既往感染有重要的提示價值，但不能反映真實結果，存在三種可能，應進一步複檢確證。
목적：대 ELISA 법검측 HBV-M 정항 HBc 음성、항 HBe 양성결과적과학평개。방법ELISA 법초검 HBV-M 정항 HBc 음성，항 HBe 양성양본105례，이 CMIA 법위삼고방법복검학증항 HBe 화항 HBc 병평개기진양성솔화가음성솔；이기 S／CO 수평불동분조평개불동수평항 HBe 화항 HBc 검측결과적진실성。결과CMIA 법복검후산생3충불동결과，즉항 HBc 양성／항 HBe 양성、항 HBc 음성／항 HBe 음성、항 HBc 양성／항 HBe 음성，분별점72.38％，21.91％화5.71％，항 HBc 가음성솔화항 HBe 진양성솔분별위78.10％화72.38％；재0.00～0.10，0.10～0.80화0.80～1.00불동 S／CO 수평내항 HBe 진양성솔분별위42.86％，88.14％화56.25％，재1.00～1.20，1.20～2.00화2.00～3.00불동 S／CO 수평내항 HBc 가음성솔분별위93.33％，96.15％화47.37％。결론ELISA 법검측 HBV-M 정항-HBc 음성／항 HBe 양성결과대HBV 기왕감염유중요적제시개치，단불능반영진실결과，존재삼충가능，응진일보복검학증。
Objective To research the reality of ELISA testing results with negative anti-HBc and positive antiHBe.Methods CMIA was carried out to retest antiHBc and antiHBe of 105 samples which were initially tested to have negative antiHBc and positive antiHBe.Results Among the 105 samples retested by CMIA,there were three different results,positive antiH-Bc with positive antiHBe,negative antiHBc with negative antiHBe and positive antiHBc with negative antiHBe,whose pro-portions were 72.38%,21.91% and 5.71% respectively;the fasle negative rates of antiHBc were 78.10% in total and 93.33%,96.15% and 47.37% in 3 subgroup with S/CO 1.00~ 1.20,1.20~2.00 and 2.00~ 3.00,respectively;the true positive rates of antiHBe were 72.38% in total and 42.86%,88.14% and 56.25% in 3 subgroups with S/CO 0.00~0.10, 0.10~0.80 and 0.80 ~ 1.00,respectively.Conclusion HBV-M results with negative antiHBc and positive antiHBe by ELISA could give suggestive value and not reflect true information about antiHBc and antiHBe with three alternatives which would be obtained through retesting by a second assay.
