CAJ | 학술논문

饲养并繁殖酸敏感离子通道1(ASIC1)基因敲除杂合子小鼠,提取小鼠尾部组织DNA,采用聚合酶链反应( PCR)方法鉴定子代小鼠基因型. ASIC1 基因敲除小鼠的繁育和鉴定均获得成功,子代小鼠基因型分别为杂合子( ASIC1+/-)、纯合子( ASIC1-/ -)和野生型( ASIC1+/ +).
사양병번식산민감리자통도1(ASIC1)기인고제잡합자소서,제취소서미부조직DNA,채용취합매련반응( PCR)방법감정자대소서기인형. ASIC1 기인고제소서적번육화감정균획득성공,자대소서기인형분별위잡합자( ASIC1+/-)、순합자( ASIC1-/ -)화야생형( ASIC1+/ +).
To breed and identify acid sensing ion channel 1(ASIC1) gene knockout mice, so as to lay the founda-tion for studying ASIC1 protein. The heterozygote mice were bred and reproduced. Genome DNA extracted from the murine tail was subjected to PCR test for genotype identification. Breeding and reproducing of ASIC1 knockout mice were both successful,and the genotypes of the offspring mice were heterozygous( ASIC1+/ -) ,homozygous( ASIC1-/ -) ,and wild-type( ASIC1+/ +) . Appropriate methods of breeding,reproducing and identifying can effective-ly obtain ASIC1-/ - mice.