CAJ | 학술논문

目的研究电针三阴交对自然围绝经期大鼠卵巢雌二醇（ E2）和大脑皮层神经递质的影响。方法本实验以自然衰老大鼠为模型，将大鼠以随机数字法分为治疗组（电针组）、模型组（对照组）、正常组（青年组），每组7只。治疗组大鼠予三阴交电针治疗，隔日一次，共治疗15次。其他两组大鼠进行常规饲养，不进行针刺，隔日抓取。采用高效液相色谱－配以电化学检测法（ HPLC-ECD）测定各组大脑皮层中神经递质的含量，酶联免疫吸附法测定卵巢E2含量。结果 E2模型组的E2含量比正常组显著降低（P＜0．01），治疗组的E2含量比模型组显著升高（P＜0．01）。与正常组比较，模型组大鼠多巴胺（DA）含量下降不明显（P＞0．05），NE的含量显著下降（P＜0．01），5-羟色胺（5-HT）的含量升高显著（P＜0．01）；模型组的5-HT／去甲肾上腺素（NE）值显著升高（P＜0．01）。与模型组比较，治疗组大鼠的DA含量升高显著（P＜0．01），NE的含量也明显升高（P＜0．05），5-HT的含量显著降低（P＜0．01），治疗组的5-HT／NE值降低显著（P＜0．01）。结论电针三阴交可以有效调节自然衰老围绝经期大鼠卵巢E2和大脑皮层神经递质的含量。
목적연구전침삼음교대자연위절경기대서란소자이순（ E2）화대뇌피층신경체질적영향。방법본실험이자연쇠로대서위모형，장대서이수궤수자법분위치료조（전침조）、모형조（대조조）、정상조（청년조），매조7지。치료조대서여삼음교전침치료，격일일차，공치료15차。기타량조대서진행상규사양，불진행침자，격일조취。채용고효액상색보－배이전화학검측법（ HPLC-ECD）측정각조대뇌피층중신경체질적함량，매련면역흡부법측정란소E2함량。결과 E2모형조적E2함량비정상조현저강저（P＜0．01），치료조적E2함량비모형조현저승고（P＜0．01）。여정상조비교，모형조대서다파알（DA）함량하강불명현（P＞0．05），NE적함량현저하강（P＜0．01），5-간색알（5-HT）적함량승고현저（P＜0．01）；모형조적5-HT／거갑신상선소（NE）치현저승고（P＜0．01）。여모형조비교，치료조대서적DA함량승고현저（P＜0．01），NE적함량야명현승고（P＜0．05），5-HT적함량현저강저（P＜0．01），치료조적5-HT／NE치강저현저（P＜0．01）。결론전침삼음교가이유효조절자연쇠로위절경기대서란소E2화대뇌피층신경체질적함량。
Objective To investigate the efficacy of electro-acupuncturing ( EA) Sanyinjiao on estradiol and cerebral neurotransmitters in natural premenopausal rats.Methods The natural aging rats was slected as animal model. The rats were randomly divided into treatment group ( EA group ) , model group ( control group ) , the normal group ( youth group) ,7 rats in each group.Rats in EA group were treated by Sanyinjiao acupuncture every other day,and total-ly 15 times.Other groups of rats were feed routine and crawled every other day without acupuncture.Cortex neurotrans-mitter levels of each group were determined by HPLC with electrochemical detection ( HPLC-ECD) .The contents of es-tradiol of each group were detected by enzyme-linked immunosorbent assay.Results Estradiol level of model group was significantly lower than that of the normal groups(P<0.01).Compared with model group,Estradiol was increased sig-nificantly in treatment group (P<0.01).Compared with normal group,DA was decreased in model group,but no statis-tical significance (P>0.05),while the NE was decreased and 5-HT increasing significantly (P<0.01).The ratio of 5-HT/NE was increased significantly in model group(P<0.01).Compared with the model group,DA and NE of treat-ment group were significantly increased (P<0.01,P<0.05),but 5-HT was significantly decreased (P<0.01).The ratio of 5-HT/NE was decreased significantly in treatment group when compared with model group (P<0.01).Conclu-sion EA Sanyinjiao can effectively regulate the ovarian estradiol and cerebral cortex neurotransmitters of natural aging perimenopausal rats.There are significant correlation between estradiol and the cerebral cortex neurotransmitters inclu-ding DA,NE and 5-HT.