CAJ | 학술논문

目的：探讨香烟暴露诱导的肺气肿小鼠肺组织中白介素-17A（IL-17A）以及白介素-22（IL-22）的变化以及戒烟、N-乙酰半胱氨酸（NAC）对 IL-17A 和 IL-22的影响。方法采用香烟暴露法建立小鼠肺气肿模型，给予戒烟及 NAC 灌胃干预。采用 ELISA 法检测肺匀浆及肺泡灌洗液（BALF）中IL-17A 和 IL-22浓度。HE 染色观察肺组织病理学变化。结果香烟暴露可使小鼠肺组织发生肺气肿样变化。与正常对照组相比，肺匀浆及 BALF 中，香烟暴露后 IL-17A 及 IL-22水平升高（P ＜0．05）。与香烟暴露组比较，戒烟及 NAC 灌胃后 IL-17A 及 IL-22水平下降（P ＜0．05）。肺匀浆及 BALF中 IL-22与 IL-17A 的比值逐渐降低。结论 IL-17A 及 IL-22与香烟暴露导致的小鼠肺组织中慢性炎症的发生有关。戒烟及 NAC 处理对吸烟导致的 IL-17A 及 IL-22变化有一定干预作用。
목적：탐토향연폭로유도적폐기종소서폐조직중백개소-17A（IL-17A）이급백개소-22（IL-22）적변화이급계연、N-을선반광안산（NAC）대 IL-17A 화 IL-22적영향。방법채용향연폭로법건립소서폐기종모형，급여계연급 NAC 관위간예。채용 ELISA 법검측폐균장급폐포관세액（BALF）중IL-17A 화 IL-22농도。HE 염색관찰폐조직병이학변화。결과향연폭로가사소서폐조직발생폐기종양변화。여정상대조조상비，폐균장급 BALF 중，향연폭로후 IL-17A 급 IL-22수평승고（P ＜0．05）。여향연폭로조비교，계연급 NAC 관위후 IL-17A 급 IL-22수평하강（P ＜0．05）。폐균장급 BALF중 IL-22여 IL-17A 적비치축점강저。결론 IL-17A 급 IL-22여향연폭로도치적소서폐조직중만성염증적발생유관。계연급 NAC 처리대흡연도치적 IL-17A 급 IL-22변화유일정간예작용。
Objective To investigate the changes of interleukin-17A(IL-17A)and interleukin-22(IL-22)in the lung of cigarette smoke exposed mice and the effect of smoking cessation and N -acetylcysteine (NAC )treatment . Methods BALB /c mice in experimental groups were exposed to cigarette smoke.Then the smoke-exposure was stopped and mice were treated with NAC gavage.The mice were executed 1,2,and 3 months after smoking cessa-tion.Lung tissue sample and bronchoalveolar lavage fluid(BALF)were collected.HE staining was used to observe the pathologic changes of the lung and ELISA was used to measure the level of IL-17A and IL-22.Results Con-siderable emphysematous changes was found in the lung of mice exposed to cigarette smoke.Compared with the controls,the level of IL-17A and IL-22 elevated remarkably in pulmonary tissue and BALF after smoking exposure and declined gradually after smoking cessation.Additional NAC gavage treatment enhanced the decline tendency. Conclusion IL-17A and IL-22 might play a complex role in the chronic inflammatory changes of lung in mice ex-posed to cigarette smoke.