CAJ | 학술논문

目的研究糖皮质激素治疗重症肌无力( MG)患者外周血CD4 +CD25 +调节性 T 细胞( Treg )中 Foxp3 和胞内CTLA-4的表达水平的变化. 方法收集MG患者34例,分为非治疗组8例和糖皮质激素治疗组26 例. 另将20 例健康者设为对照组. 采用流式细胞仪检测分析患者和健康者外周血CD4 +CD25 +Treg细胞Foxp3和胞内CTLA-4的变化.结果 ① MG非治疗组患者外周血 CD4 +CD25 +Treg细胞Foxp3和胞内CTLA-4水平较对照组显著下降( P<0. 05 );②糖皮质激素治疗能显著提升CD4 +CD25 +Treg细胞Foxp3和胞内CTLA-4表达比例( P <0. 05 ) ,但仍低于对照组( P <0. 05);③ CD4 +CD25 +Treg细胞Foxp3和胞内CTLA-4表达呈正相关性(r=0. 870,P<0. 001). 结论 MG患者外周血CD4 +CD25 +Treg Foxp3和胞内CTLA-4呈低表达,糖皮质激素治疗能显著提升Foxp3和胞内CTLA-4表达水平,但尚未达正常者免疫状态.
목적 연구당피질격소치료중증기무력( MG)환자외주혈CD4 +CD25 +조절성 T 세포( Treg )중 Foxp3 화포내CTLA-4적표체수평적변화. 방법 수집MG환자34례,분위비치료조8례화당피질격소치료조26 례. 령장20 례건강자설위대조조. 채용류식세포의검측분석환자화건강자외주혈CD4 +CD25 +Treg세포Foxp3화포내CTLA-4적변화.결과 ① MG비치료조환자외주혈 CD4 +CD25 +Treg세포Foxp3화포내CTLA-4수평교대조조현저하강( P<0. 05 );②당피질격소치료능현저제승CD4 +CD25 +Treg세포Foxp3화포내CTLA-4표체비례( P <0. 05 ) ,단잉저우대조조( P <0. 05);③ CD4 +CD25 +Treg세포Foxp3화포내CTLA-4표체정정상관성(r=0. 870,P<0. 001). 결론 MG환자외주혈CD4 +CD25 +Treg Foxp3화포내CTLA-4정저표체,당피질격소치료능현저제승Foxp3화포내CTLA-4표체수평,단상미체정상자면역상태.
Objective To study the expression alteration of Foxp3 and intracellular CTLA-4 of CD4 +CD25 +regula-tory T cells in peripheral blood of myasthenia gravis patients under glucocorticoids treatment. Methods 34 patients with myasthenia gravis were selected, and subgrouped with no treatment group(8 cases) or glucocorticoids treat-ment group(26 cases) . Another 20 healthy subjects were set as the control group. 6-color flow cytometry was used to analyze the expression of Foxp3 and intracellular CTLA-4 of CD4 +CD25 + regulatory T cells in their peripheral blood of participants. Results ①Compared with the control group, the level of Foxp3 and intracellular CTLA-4 of CD4 +CD25 +regulatory T cells in peripheral blood of in no treatment group were significantly decreased ( P <0. 05 ) . ② the level of Foxp3 and intracellular CTLA-4 of CD4 +CD25 +regulatory T cells in peripheral blood of pa-tients with glucocorticoids treatment group were significantly higher than that of no treatment group(P<0. 05), but were still lower than that of the control group(P<0. 05). ③Foxp3 expression was positively correlated with intra-cellular CTLA-4 expression in CD4 +CD25 +regulatory T cells of patients with myasthenia gravis ( r=0. 870 , P<0. 001 ) . Conclusion Low expression of Foxp3 and intracellular CTLA-4 of CD4 +CD25 + regulatory T cells in pe-ripheral blood is present in patients with myasthenia gravis, glucocorticoids could significantly increase the expres-sion of Foxp3 and intracellular CTLA-4, however, which still do not reach the immune state of healthy subjects.