CAJ | 학술논문

目的探讨人参皂苷Rg3对鼻咽癌放疗患者细胞免疫功能的影响. 方法选取鼻咽癌放疗患者共50例为研究对象,按随机数字表法分为对照组、Rg3 组,每组25 例. 放疗结束后,分离各组患者外周血淋巴细胞. 采用MTT法检测淋巴细胞增殖,流式细胞仪检测淋巴细胞亚群,间接ELISA法检测IgG、IgM抗体水平及Th1/Th2细胞因子表达.结果人参皂苷Rg3可促进鼻咽癌放疗患者淋巴细胞增殖,上调CD4 +、CD4 + /CD8 +、IgG、IgM、IL-2,下调CD8 +、IL-6,并呈现剂量依赖性. 结论人参皂苷Rg3可显著增强鼻咽癌放疗患者外周血淋巴细胞免疫功能,提示人参皂苷Rg3可作为潜在免疫增强剂,拮抗鼻咽癌放疗所致的免疫抑制.
목적 탐토인삼조감Rg3대비인암방료환자세포면역공능적영향. 방법 선취비인암방료환자공50례위연구대상,안수궤수자표법분위대조조、Rg3 조,매조25 례. 방료결속후,분리각조환자외주혈림파세포. 채용MTT법검측림파세포증식,류식세포의검측림파세포아군,간접ELISA법검측IgG、IgM항체수평급Th1/Th2세포인자표체.결과 인삼조감Rg3가촉진비인암방료환자림파세포증식,상조CD4 +、CD4 + /CD8 +、IgG、IgM、IL-2,하조CD8 +、IL-6,병정현제량의뢰성. 결론 인삼조감Rg3가현저증강비인암방료환자외주혈림파세포면역공능,제시인삼조감Rg3가작위잠재면역증강제,길항비인암방료소치적면역억제.
Objective To evaluate the effect of ginsenoside-Rg3 on cellular immune function of nasopharyngeal carcinoma patients with radiotherapy. Methods 50 nasopharyngeal carcinoma patients with radiotherapy were ran-domly divided into Rg3 group ( n=25 ) and control group ( n=25 ) . After the radiotherapy, the peripheral blood lymphocy of each group were collected. MTT method was used to detect lymphocyte proliferation. Lymphocyte sub-group was detected by Flow Cytometry/financal capacity model. IgG,IgM and Th1/Th2 cytokines was detected by ELISA. Results Ginsenoside Rg3 could promote the proliferation of lymphocytes in patients with nasopharyngeal carcinoma radiotherapy,upregulation of CD4 +,CD4 +/CD8 +,IgG,IgM,IL-2,downregulation of CD8 +,IL-6,and showed dose dependent. Conclusion Rg3 ginsenosides can significantly enhance the peripheral blood lymphocyte immune function of nasopharyngeal carcinoma patients with radiotherapy. Rg3 ginsenosides may be a potential im-mune immunosuppression caused by nasopharyngeal carcinoma radiotherapy enhancer antagonism.