CAJ | 학술논문

目的：研究千金子甾醇在大鼠肠道内的吸收情况以及P-糖蛋白（ P-gp）和多药耐药相关蛋白（ MRP2）对千金子甾醇肠吸收的影响。方法采用大鼠在体单向肠灌流模型，运用高效液相色谱法测定十二指肠、空肠、回肠、结肠灌流液中千金子甾醇的含量，计算吸收速率常数（ Ka ）和表观渗透系数（Papp）。结果千金子甾醇在大鼠结肠的Ka 及Papp最高（P＜0．05）。加入P-gp抑制剂盐酸维拉帕米后，千金子甾醇在结肠段的Ka 及Papp显著增加；而加入MRP2抑制剂吲哚美辛后，千金子甾醇在大鼠结肠段的Ka及Papp普遍降低。结论千金子甾醇在肠道中的主要吸收部位为结肠，推测千金子甾醇可能为P-gp的底物，而非MRP2的底物。
목적：연구천금자치순재대서장도내적흡수정황이급P-당단백（ P-gp）화다약내약상관단백（ MRP2）대천금자치순장흡수적영향。방법채용대서재체단향장관류모형，운용고효액상색보법측정십이지장、공장、회장、결장관류액중천금자치순적함량，계산흡수속솔상수（ Ka ）화표관삼투계수（Papp）。결과천금자치순재대서결장적Ka 급Papp최고（P＜0．05）。가입P-gp억제제염산유랍파미후，천금자치순재결장단적Ka 급Papp현저증가；이가입MRP2억제제신타미신후，천금자치순재대서결장단적Ka급Papp보편강저。결론천금자치순재장도중적주요흡수부위위결장，추측천금자치순가능위P-gp적저물，이비MRP2적저물。
Objective To study the characteristic of absorption of euphorbia factor L1 in intestine of rats, and to observe the effects of P-glycoprotein(P-gp)and multidrug resistance-associated protein(MRP2)on intestinal absorption of euphorbia factor L1 .Methods The contents of euphorbia factor L1 of intestinal perfusion fluid of duodenum, jejunum, ileum and colon in the rats in situ single-pass intestinal perfusion model were determined by using HPLC.The drug absorption rate constant( Ka ) and the apparent absorp-tion coefficient( Papp ) in four intestinal regions were calculated.Results Ka and Papp of euphorbia factor L1 at colon were the highest of the whole rat intestine.Significant increase of Ka and Papp showed at rat colon when co-perfused with verapamil hydrochloride, by contrast, decrease of Ka and Papp found when co-perfused with indomethacin.Conclusion We inferred that verapamil hydrochloride be the substrate of P-gp and that indomethacin be not the substrate of MRP2 .