CAJ | 학술논문

目的：研究加味生脉饮含药血清对非小细胞肺癌细胞迁移、黏附的影响，并探讨其分子机制。方法以生药量18 g／kg灌胃家兔，制备加味生脉饮含药血清，同容积生理盐水灌胃制备空白血清。体外培养细胞，收集对数期生长的细胞，设10％空白血清组，2．5％、5％、10％含药血清组4个组。 Transwell和黏附实验分别检测加味生脉饮含药血清对非小细胞肺癌细胞迁移及黏附的影响；RT-PCR测定经含药血清处理后细胞中N钙连素（ N-cad）及波形蛋白（ vimentin）基因表达的变化。结果随着含药血清浓度的增高，迁移到膜下表面的细胞数减少，5％和10％含药血清组迁移率仅为25．64％和13．31％（P＜0．01）。随着含药血清浓度增高和作用时间延长，异质黏附率降低，且黏附率和血清浓度及作用时间呈负相关；同时N-cad、vimentin基因的表达量下降，呈浓度与时间相关性。结论加味生脉饮含药血清对非小细胞肺癌细胞有抑制迁移和降低异质黏附作用，其分子机制与影响上皮间质转化有关。
목적：연구가미생맥음함약혈청대비소세포폐암세포천이、점부적영향，병탐토기분자궤제。방법이생약량18 g／kg관위가토，제비가미생맥음함약혈청，동용적생리염수관위제비공백혈청。체외배양세포，수집대수기생장적세포，설10％공백혈청조，2．5％、5％、10％함약혈청조4개조。 Transwell화점부실험분별검측가미생맥음함약혈청대비소세포폐암세포천이급점부적영향；RT-PCR측정경함약혈청처리후세포중N개련소（ N-cad）급파형단백（ vimentin）기인표체적변화。결과수착함약혈청농도적증고，천이도막하표면적세포수감소，5％화10％함약혈청조천이솔부위25．64％화13．31％（P＜0．01）。수착함약혈청농도증고화작용시간연장，이질점부솔강저，차점부솔화혈청농도급작용시간정부상관；동시N-cad、vimentin기인적표체량하강，정농도여시간상관성。결론가미생맥음함약혈청대비소세포폐암세포유억제천이화강저이질점부작용，기분자궤제여영향상피간질전화유관。
Objective To study the effects and mechanism of serum containing modified Shengmai liquid ( MSL) on migration and adhesion of non small cell lung cancer ( NSCLC) cells.Methods Rabbit ser-um containing MSL was prepared by intragastric administration ( i.g.) with MSL at the dose of 18 g/kg and control serum was prepared by i.g.normal saline.NSCLC cells were cultured, and those in logarith-mic growth phase collected were divided into four groups:10%control serum(CS) group, 2.5%, 5%, 10%MSL serum group.Migration cells were detected by using Transwell invasion assay, and adhension rate was measured by using matrigel adhensive assay.The expressions of N-cad and vimentin were de-termined by RT-PCR.Results The number of migration cells decreased as the concentration of serum containg MSL increased:migration rate of 5%MSL group was 25.64%and that of 10%MSL group was 13.31%(P<0.01).As the concentration of serum containing MSL increased and the duration of treat-ment prolonged, heterogeneous adhesion rate decreased and was negatively correlated with serum concen-tration and duration;while the gene expressions of N-cad and vimentin decreased in dose-and time-de-pendent manner.Conclusion The mechanism of inhabitation migration and reduction heterogeneity ad-hension of MSL on NSCLC cells would be related to the epithelial-mesenchymal transition.