CAJ | 학술논문

目的比较 miRNA-140 和 MMP-3 在骨性关节炎( OA)组与非OA组关节滑液中的表达,探究miRNA-140 和MMP-3与OA的相关性. 方法采用实时定量PCR技术检测miRNA-140、MMP-3在OA组(n=65)与非OA组(n=65)关节滑液中的表达水平,并运用 Spearman 相关分析分析miRNA-140与MMP-3的相关性. 结果组间比较显示OA 组miRNA-140的表达显著低于非OA组(P<0. 05),MMP-3的表达明显高于非OA组( P<0. 05 ). OA组内各因子之间相关分析显示,miRNA-140 和 MMP-3 呈显著负相关性( r=-0. 693、-0. 720,P<0. 05). 结论相对于非OA病变,OA组关节滑液中miRNA-140 低表达,MMP-3高表达,两者之间呈负相关性.
목적 비교 miRNA-140 화 MMP-3 재골성관절염( OA)조여비OA조관절활액중적표체,탐구miRNA-140 화MMP-3여OA적상관성. 방법 채용실시정량PCR기술검측miRNA-140、MMP-3재OA조(n=65)여비OA조(n=65)관절활액중적표체수평,병운용 Spearman 상관분석분석miRNA-140여MMP-3적상관성. 결과 조간비교현시OA 조miRNA-140적표체현저저우비OA조(P<0. 05),MMP-3적표체명현고우비OA조( P<0. 05 ). OA조내각인자지간상관분석현시,miRNA-140 화 MMP-3 정현저부상관성( r=-0. 693、-0. 720,P<0. 05). 결론 상대우비OA병변,OA조관절활액중miRNA-140 저표체,MMP-3고표체,량자지간정부상관성.
Objective To exploring the correlation between miRNA-140 and MMP-3 in osteoarthritis ( OA ) , this study compared the OA group with non-OA group over the expression of MMP-3 and miRNA-140 in synovial fluid. Methods Using Real-time quantitative PCR to detect the expression levels of miRNA-140 , MMP-3 in synovial flu-id both OA group(n=65) and non-OA group(n=65), then exploring the relationship between miRNA-140 and MMP-3 by using Spearman correlation analysis software. Results The expression of miRNA-140 in OA group was significantly lower than non-OA group ( P<0. 05 ) , while the expression of MMP-3 in OA group was significantly higher than non-OA group ( P<0. 05 ) . miRNA-140 and MMP-3 had a significant negative correlation in OA group by using Spearman correlation analysis software, the correlation coefficient(r = -0. 693、-0. 720, P <0. 05). Conclusions Relative to non-OA lesions, miRNA-140 shows low expression in OA synovial fluid group, while MMP-3 in the OA group is highly expressed, both showed a significant negative correlation in OA group and non-OA group.