安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
Acta Universitatis Medicinalis Anhui
2015年
11期
1556-1559
,共4页
程光存%严中亚%李春生%严宇%韦晓勇%李建华%汤丹丹%程光明%董桂福%姜波%蔡燕
程光存%嚴中亞%李春生%嚴宇%韋曉勇%李建華%湯丹丹%程光明%董桂福%薑波%蔡燕
정광존%엄중아%리춘생%엄우%위효용%리건화%탕단단%정광명%동계복%강파%채연
羟基磷灰石薄膜%犬血管内皮细胞%细胞培养%组织工程
羥基燐灰石薄膜%犬血管內皮細胞%細胞培養%組織工程
간기린회석박막%견혈관내피세포%세포배양%조직공정
hydroxyapatite film%vascular endothelial cells of dog%cell culture%tissue engineering
目的：通过体外内皮化和体内内皮化，探讨沉积胶原包埋羟基磷灰石（HA）涂层人工机械瓣膜的可行性及其在人工机械瓣膜支架体内的再细胞化能力。方法制备犬血管内皮细胞（VEC）悬液，接种在胶原包埋 HA 涂层人工机械瓣膜材料上，置培养箱内分组培养如下：①37℃孵箱中静态培养2周；②动态旋转培养装置中培养2周，比较两组 VEC 分泌一氧化氮（NO）、前列环素（PGI2）水平；再分别将人工机械瓣膜植入犬右心房，术后6周，取出部分材料，扫描电子显微镜（SEM）下观察 VEC 在 HA 材料上的附着情况，了解 HA支架材料在体内的再细胞化能力。结果与静态系统相比，人工机械瓣膜在动态旋转系统构建胶原包埋 HA 涂层 VEC分泌 NO 、PGI2水平显著升高（P ＜0．05）；在犬右心房人工机械瓣膜取出材料中，动态旋转系统构建胶原包埋 HA 涂层VEC 层均匀分布，并有心脏内皮细胞附着，而静态培养系统中 VEC 层分布不均匀，胶原包埋 HA 涂层有心脏血管内皮细胞附着，而单纯人工机械瓣薄膜表面出现较多的血栓形成。结论动态旋转系统中构建的组织工程化瓣膜模型中， VEC 可能成为组织工程化机械瓣膜材料；胶原包埋的 HA 涂层人工机械瓣膜有利于 VEC 的黏附和生长。
目的：通過體外內皮化和體內內皮化，探討沉積膠原包埋羥基燐灰石（HA）塗層人工機械瓣膜的可行性及其在人工機械瓣膜支架體內的再細胞化能力。方法製備犬血管內皮細胞（VEC）懸液，接種在膠原包埋 HA 塗層人工機械瓣膜材料上，置培養箱內分組培養如下：①37℃孵箱中靜態培養2週；②動態鏇轉培養裝置中培養2週，比較兩組 VEC 分泌一氧化氮（NO）、前列環素（PGI2）水平；再分彆將人工機械瓣膜植入犬右心房，術後6週，取齣部分材料，掃描電子顯微鏡（SEM）下觀察 VEC 在 HA 材料上的附著情況，瞭解 HA支架材料在體內的再細胞化能力。結果與靜態繫統相比，人工機械瓣膜在動態鏇轉繫統構建膠原包埋 HA 塗層 VEC分泌 NO 、PGI2水平顯著升高（P ＜0．05）；在犬右心房人工機械瓣膜取齣材料中，動態鏇轉繫統構建膠原包埋 HA 塗層VEC 層均勻分佈，併有心髒內皮細胞附著，而靜態培養繫統中 VEC 層分佈不均勻，膠原包埋 HA 塗層有心髒血管內皮細胞附著，而單純人工機械瓣薄膜錶麵齣現較多的血栓形成。結論動態鏇轉繫統中構建的組織工程化瓣膜模型中， VEC 可能成為組織工程化機械瓣膜材料；膠原包埋的 HA 塗層人工機械瓣膜有利于 VEC 的黏附和生長。
목적：통과체외내피화화체내내피화，탐토침적효원포매간기린회석（HA）도층인공궤계판막적가행성급기재인공궤계판막지가체내적재세포화능력。방법제비견혈관내피세포（VEC）현액，접충재효원포매 HA 도층인공궤계판막재료상，치배양상내분조배양여하：①37℃부상중정태배양2주；②동태선전배양장치중배양2주，비교량조 VEC 분비일양화담（NO）、전렬배소（PGI2）수평；재분별장인공궤계판막식입견우심방，술후6주，취출부분재료，소묘전자현미경（SEM）하관찰 VEC 재 HA 재료상적부착정황，료해 HA지가재료재체내적재세포화능력。결과여정태계통상비，인공궤계판막재동태선전계통구건효원포매 HA 도층 VEC분비 NO 、PGI2수평현저승고（P ＜0．05）；재견우심방인공궤계판막취출재료중，동태선전계통구건효원포매 HA 도층VEC 층균균분포，병유심장내피세포부착，이정태배양계통중 VEC 층분포불균균，효원포매 HA 도층유심장혈관내피세포부착，이단순인공궤계판박막표면출현교다적혈전형성。결론동태선전계통중구건적조직공정화판막모형중， VEC 가능성위조직공정화궤계판막재료；효원포매적 HA 도층인공궤계판막유리우 VEC 적점부화생장。
Objective Through endothelialization in vitro and in vivo,to explore the feasibility of the collagen de-posit to embed hydroxyapatite (HA)coating of artificial mechanical valve and the ability of recellularization in arti-ficial mechanical valve scaffolds.Methods To prepare canine vascular endothelial cells (VECs)suspension,seed in collagen embedding HA coating artificial mechanical valve material.The VECs were cultured in grouping as fol-lows:① 37 ℃ static training in the incubator ② dynamic rotation cultivation device.After two weeks,to compare the secretion levels of nitric oxide (NO),prostacyclin (PGI2 )in VECs of the two groups.And then,the artificial mechanical valves were implanted in right atrium of dogs respectively.After six weeks of surgery,some materials were taken out from dogs right atrium to scan by electron microscope and observe the adhesion situation of VECs on HA material and explore the recellularization ability of HA scaffold material in vivo.Results Compared with the static culture system,the secretion levels of NO and PGI2 of building collagen embedding HA coating VEC of artifi-cial mechanical valve in dynamic rotation system were significantly elevated (P <0.05).In the materials taken from dog right atrial mechanical valve,collagen embedding HA coating VEC layer was distributed evenly and had cardiac endothelial cells adhesion in the dynamic rotation cuture system.The distribution of VECs layer was uneven in static culture system.There was collagen embedding HA coating with heart endothelial cells adhesion too.There were some thrombosis on pure artificial mechanical valve film surface.Conclusion To construct tissue-engineered heart valves model in dynamic rotation culture system,VECs might become a tissue-engineered mechanical valve materials.HA coating of artificial mechanical valve embeded by collagen is advantageous to the adhesion and growth of VECs.
