CAJ | 학술논문

目的：研究Hedgehog信号通路在骨髓间充质干细胞( BMSCs)来源exosome介导小鼠4T1乳腺癌细胞发展过程中的作用。方法使用梯度离心分离法、差速贴壁培养方法及超速离心法分离C57BL/6小鼠BMSCs及其exosome,使用MTT法、细胞划痕实验及western blot技术分析exosome干预前后4T1癌细胞增殖、迁移和侵袭能力以及Hedgehog信号通路相关蛋白表达情况,之后使用GANT61(Hedgehog信号通路阻断剂)验证Hedgehog信号通路在BM-SCs来源的exosome介导乳腺癌细胞增殖、迁移和侵袭过程中的作用。结果 BMSCs来源的exosome显著上调了4T1细胞Hedgehog信号通路,exosome显著增加了4T1细胞的增殖及迁移、侵袭能力,而这一作用被GANT61所削减。结论 BMSCs来源的exosome能够通过上调Hedgehog信号通路增加4T1小鼠乳腺癌细胞的增殖、迁移及侵袭能力。
목적：연구Hedgehog신호통로재골수간충질간세포( BMSCs)래원exosome개도소서4T1유선암세포발전과정중적작용。방법사용제도리심분리법、차속첩벽배양방법급초속리심법분리C57BL/6소서BMSCs급기exosome,사용MTT법、세포화흔실험급western blot기술분석exosome간예전후4T1암세포증식、천이화침습능력이급Hedgehog신호통로상관단백표체정황,지후사용GANT61(Hedgehog신호통로조단제)험증Hedgehog신호통로재BM-SCs래원적exosome개도유선암세포증식、천이화침습과정중적작용。결과 BMSCs래원적exosome현저상조료4T1세포Hedgehog신호통로,exosome현저증가료4T1세포적증식급천이、침습능력,이저일작용피GANT61소삭감。결론 BMSCs래원적exosome능구통과상조Hedgehog신호통로증가4T1소서유선암세포적증식、천이급침습능력。
Objective To investigate the roles of Hedgehog signaling pathway in the BMSCs derived exosome-in-duced progression of 4T1 mouse breast cancer cells. Methods Prepare the BMSCs and exosome by gradient centrifugation separation, differential adhesion method and ultracentrifugation method;use the MTT test, cell scratch test and western blot to examine the effect of BMSCs-exosome on the proliferation, migration and invasion ability of 4T1 cells, then use the GANT61, an inhibitor of Hedgehog signaling pathway, to test the mechanism of exosome-induced changes in the 4T1 cells. Results BMSCs-exosome significantly up-regulated the Hedgehog signaling pathway of the 4T1 cells. The exosome signifi-cantly increased the proliferation, migaration and invasion ability of 4T1 cells in vitro, and this effect was abolished by GANT61. Conclusions BMSCs derived exosome can promote the proliferation, migration and invasion ability by upregu-late Hedgehog signaling pathway.