中国骨与关节杂志
中國骨與關節雜誌
중국골여관절잡지
Chinese Journal of Bone and Joint
2015年
9期
701-705
,共5页
李帅%郜勇%王琨%刘伟%宋雨%杨述华%杨操
李帥%郜勇%王琨%劉偉%宋雨%楊述華%楊操
리수%고용%왕곤%류위%송우%양술화%양조
骨肉瘤%细胞凋亡%基因表达调控%RNA,肿瘤%微小 RNA-383
骨肉瘤%細胞凋亡%基因錶達調控%RNA,腫瘤%微小 RNA-383
골육류%세포조망%기인표체조공%RNA,종류%미소 RNA-383
Osteosarcoma%Apoptosis%Gene expression regulation%RNA,neoplasm%MicroRNA-383
目的：观察微小 RNA ( miR )-383对骨肉瘤细胞生物学特性的影响。方法脂质体转染骨肉瘤细胞( U-2OS )，检测对细胞凋亡、增殖的影响，并检测细胞迁移侵袭能力、克隆形成能力的改变。结果细胞凋亡情况：空白对照组、无义序列组和实验组细胞的凋亡率分别为(8.56±1.45)%、(12.6l±1.37)%、(33.20±2.59)%，差异有统计学意义(P＜0.05)；细胞迁移实验：空白对照组、无义序列组和实验组分别为(82±5)个、(68±3)个和(45±7)个，差异有统计学意义(P＜0.05)；细胞迁移与侵袭实验：空白对照组、无义序列组和实验组细胞穿膜数分别为(82±5)个、(68±3)个和(45±7)个，以及(35±9)个、(32±4)个和(13±5)个，差异有统计学意义(P＜0.05)；克隆形成实验：空白对照组、无义序列组和实验组克隆数分别为(12.7±4.1)个、(12.3±3.6)个和(6.4±2.7)个。结论抑制 miR-383使骨肉瘤细胞凋亡增加，使细胞增殖、侵袭与迁移及克隆形成受到抑制。
目的：觀察微小 RNA ( miR )-383對骨肉瘤細胞生物學特性的影響。方法脂質體轉染骨肉瘤細胞( U-2OS )，檢測對細胞凋亡、增殖的影響，併檢測細胞遷移侵襲能力、剋隆形成能力的改變。結果細胞凋亡情況：空白對照組、無義序列組和實驗組細胞的凋亡率分彆為(8.56±1.45)%、(12.6l±1.37)%、(33.20±2.59)%，差異有統計學意義(P＜0.05)；細胞遷移實驗：空白對照組、無義序列組和實驗組分彆為(82±5)箇、(68±3)箇和(45±7)箇，差異有統計學意義(P＜0.05)；細胞遷移與侵襲實驗：空白對照組、無義序列組和實驗組細胞穿膜數分彆為(82±5)箇、(68±3)箇和(45±7)箇，以及(35±9)箇、(32±4)箇和(13±5)箇，差異有統計學意義(P＜0.05)；剋隆形成實驗：空白對照組、無義序列組和實驗組剋隆數分彆為(12.7±4.1)箇、(12.3±3.6)箇和(6.4±2.7)箇。結論抑製 miR-383使骨肉瘤細胞凋亡增加，使細胞增殖、侵襲與遷移及剋隆形成受到抑製。
목적：관찰미소 RNA ( miR )-383대골육류세포생물학특성적영향。방법지질체전염골육류세포( U-2OS )，검측대세포조망、증식적영향，병검측세포천이침습능력、극륭형성능력적개변。결과세포조망정황：공백대조조、무의서렬조화실험조세포적조망솔분별위(8.56±1.45)%、(12.6l±1.37)%、(33.20±2.59)%，차이유통계학의의(P＜0.05)；세포천이실험：공백대조조、무의서렬조화실험조분별위(82±5)개、(68±3)개화(45±7)개，차이유통계학의의(P＜0.05)；세포천이여침습실험：공백대조조、무의서렬조화실험조세포천막수분별위(82±5)개、(68±3)개화(45±7)개，이급(35±9)개、(32±4)개화(13±5)개，차이유통계학의의(P＜0.05)；극륭형성실험：공백대조조、무의서렬조화실험조극륭수분별위(12.7±4.1)개、(12.3±3.6)개화(6.4±2.7)개。결론억제 miR-383사골육류세포조망증가，사세포증식、침습여천이급극륭형성수도억제。
Objective To investigate effects of miRNA-383 on biological behaviors of osteosarcoma. Methods MiR-383 plasmid vectors were constructed and transfected into osteosarcoma cells. Flow cytometer was used to calculate the apoptotic rate. MTT Cell Proliferation Assay measured the cell proliferation rate. An analysis of cell migration and invasion was performed by Transwell Cell Migration / Invasion Matrigel Assay. Analysis of cell clonogenic ability was performed by Colony formation Assay.Results Apoptotic rate of control group, non-sense group and anti-miR-383 group: ( 8.56±1.45 ) %, ( 12.6l±1.37 ) %, ( 33.20±2.59 ) % with statistical signiifcance (P<0.05 ). Migration test of control group, non-sense group and anti-miR-383 group: ( 82±5 ), ( 68±3 ) and ( 45±7 ) with statistical signiifcance (P<0.05 ). Migration and invasion test of control group, non-sense group and anti-miR-383 group: ( 82±5 ), ( 68±3 ) and ( 45±7 ) compared with ( 35±9 ), ( 32±4 ) and ( 13±5 ) respectively, with statistical significance (P<0.05 ). The clonogenic units of 3 groups: ( 12.7±4.1 ), ( 12.3±3.6 ) and ( 6.4±2.7 ).Conclusions MiR-383 can promote osteosarcoma cells’ apoptosis, and restrain proliferation, migration and invasion, and the colony formation.
