现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
Journal of Modern Laboratory Medicine
2015年
4期
56-59
,共4页
脂联素%基因多态性%瑶族%冠心病
脂聯素%基因多態性%瑤族%冠心病
지련소%기인다태성%요족%관심병
adiponectin%polymorphism%yao nationality%coronary heart disease
目的：探讨广西地区瑶族冠心病患者血清中脂联素（APN）水平及其基因位点＋276 G／T 和-11377 C／G 的多态性。方法瑶族、汉族冠心病患者、瑶族和汉族正常人各100例纳入研究。采用酶联免疫吸附法（ELISA）检测研究对象血清中 APN 水平，聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测 APN 基因位点＋276G／T 和-11377C／G 的多态性。结果①两组冠心病组血清中 APN 水平明显低于两组正常组，差异有统计学意义（t ＝10.311，8.642；均 P ＝0.000）。瑶族冠心病组和汉族冠心病组血清中 APN 的差异无统计学意义（t ＝1.792，P ＝0.076）；②在 H-W 平衡检验中，四组中 APN 基因位点＋276G／T 和-11377C／G 均具有群体代表性（均 P ＞0.05）；③四组 APN＋276G／T 位点的主要型别是野生型。4组间 APN＋276G／T 位点的等位基因频率均无显著差异（均 P ＞0.05）；④冠心病组 ANP-11377C／G 基因型和等位基因频率与同民族正常组的差异有统计学意义（χ2＝8.908，P ＝0.012；χ2＝17.275，P ＝0.000）；瑶族冠心病组APN-11377C／G 基因杂合程度略低于汉族冠心病组，但差异无统计学意义（χ2＝0.363，P ＝0.834）。结论同汉族冠心病一样，血清低 APN 水平和 APN-11377C／G 位点是瑶族冠心病患者的危险因素。
目的：探討廣西地區瑤族冠心病患者血清中脂聯素（APN）水平及其基因位點＋276 G／T 和-11377 C／G 的多態性。方法瑤族、漢族冠心病患者、瑤族和漢族正常人各100例納入研究。採用酶聯免疫吸附法（ELISA）檢測研究對象血清中 APN 水平，聚閤酶鏈反應-限製性片段長度多態性（PCR-RFLP）技術檢測 APN 基因位點＋276G／T 和-11377C／G 的多態性。結果①兩組冠心病組血清中 APN 水平明顯低于兩組正常組，差異有統計學意義（t ＝10.311，8.642；均 P ＝0.000）。瑤族冠心病組和漢族冠心病組血清中 APN 的差異無統計學意義（t ＝1.792，P ＝0.076）；②在 H-W 平衡檢驗中，四組中 APN 基因位點＋276G／T 和-11377C／G 均具有群體代錶性（均 P ＞0.05）；③四組 APN＋276G／T 位點的主要型彆是野生型。4組間 APN＋276G／T 位點的等位基因頻率均無顯著差異（均 P ＞0.05）；④冠心病組 ANP-11377C／G 基因型和等位基因頻率與同民族正常組的差異有統計學意義（χ2＝8.908，P ＝0.012；χ2＝17.275，P ＝0.000）；瑤族冠心病組APN-11377C／G 基因雜閤程度略低于漢族冠心病組，但差異無統計學意義（χ2＝0.363，P ＝0.834）。結論同漢族冠心病一樣，血清低 APN 水平和 APN-11377C／G 位點是瑤族冠心病患者的危險因素。
목적：탐토엄서지구요족관심병환자혈청중지련소（APN）수평급기기인위점＋276 G／T 화-11377 C／G 적다태성。방법요족、한족관심병환자、요족화한족정상인각100례납입연구。채용매련면역흡부법（ELISA）검측연구대상혈청중 APN 수평，취합매련반응-한제성편단장도다태성（PCR-RFLP）기술검측 APN 기인위점＋276G／T 화-11377C／G 적다태성。결과①량조관심병조혈청중 APN 수평명현저우량조정상조，차이유통계학의의（t ＝10.311，8.642；균 P ＝0.000）。요족관심병조화한족관심병조혈청중 APN 적차이무통계학의의（t ＝1.792，P ＝0.076）；②재 H-W 평형검험중，사조중 APN 기인위점＋276G／T 화-11377C／G 균구유군체대표성（균 P ＞0.05）；③사조 APN＋276G／T 위점적주요형별시야생형。4조간 APN＋276G／T 위점적등위기인빈솔균무현저차이（균 P ＞0.05）；④관심병조 ANP-11377C／G 기인형화등위기인빈솔여동민족정상조적차이유통계학의의（χ2＝8.908，P ＝0.012；χ2＝17.275，P ＝0.000）；요족관심병조APN-11377C／G 기인잡합정도략저우한족관심병조，단차이무통계학의의（χ2＝0.363，P ＝0.834）。결론동한족관심병일양，혈청저 APN 수평화 APN-11377C／G 위점시요족관심병환자적위험인소。
Objective To investigate the difference of serum levels of adiponectin (APN)and its gene polymorphism +276 G/T 和-11377 C/G in Yao patients with CHD and Han CHD patients and Yao normal people in Guanxi district.Methods Yao CHD patients,Han CHD patients,Yao normal people and Han normal people,each of 100 cases were included in the study.The levels of serum adiponectin of all research object were detected by ELISA and APN+276 G/T and APN-11377 C/G genotyping were conducted by using PCR-RFLP.Results The levels of adiponectin in two groups of CHD patients were significantly lower than same race normal groups,the difference were statically significant (t=10.311,8.642,all P =0.000).The adiponectin levels of two CHD groups was not statically significant difference (t = 1.792,P =0.076);②The APN+276 G/T and APN-11377 C/G of four groups conform the Hardy-Weinberg population genetic equilibrium law (all P>0.05);③The main type of all groups APN+276 G/T was the wild type.The genotype frequencies of APN+276 G/T were no significant differences in between 4 groups (all P >0.05);④The CG and GG genotype frequency of APN-11377 C/G in two CHD groups were higher than same race normal group,the difference of Yao CHD group and Yao normal group was statically significant (χ2 =8.908,P =0.012;χ2 = 17.275,P =0.000),and the difference of Yao CHD group and Han CHD group were not statically significant (χ2 = 0.363,P = 0.834).Conclusion As with Han CHD patients,serum low APN level and APN-11377 C/G loci may be risk factors of Yao CHD patients.
